Abstract
The injection of polyacrylamide gel (Bio Gel P-4, 200–400 mesh) into the subcutaneous area of mice, induced an inflammatory reaction which was characterized by the migration of leukocytes (mainly neutrophils) from the blood vessels towards the polyacrylamide gel. A rapid protein accumulation was observed during the migration of cells towards the inflamed site. Neutrophils released some pro-inflammatory lipids (prostaglandins, leukotriene B4) and lysozyme, these products were assayed in the granuloma exudate at various times of the granuloma formation. In our experimental inflammatory model, the results suggest that neutrophils that are attracted by the polyacrylamide gel produce eicosanoids and lysozyme, which could act synergistically to potentiate cell migration and protein accumulation in the inflamed site.
Similar content being viewed by others
References
E. Fontan and R.M. Fauve,Inflammation et résistance antibactérienne, Ann. Immunol. (Inst. Pasteur)134C, 255–264 (1983).
R. Vinegar, J.F. Truax and J.L. Selph,Some quantitative temporal characteristics of carrageenan-induced pleurisy in the rats, Proc. Soc. Exp. Biol. Med.143, 711–714 (1973).
R. Vinegar, J.F. Truax, J.L. Selph and A. Voelker,Pathway of onset, development, and decay of carrageenan pleurisy in the rat. Fed. Proc.41, 2588–2594 (1982).
G.A. Higgs and J.A. Salmon,Cyclo-oxygenase products in carrageenan-induced inflammation, Prostaglandins17, 737–746 (1979).
S. Tsurufuji, S. Yoshino and K. Ohuchi,Induction of an allergic air-pouch inflammation in rats. Int. Arch. Allergy Appl. Immunol.69, 189–198 (1982).
N.S. Doherty, P. Poubelle, P. Borgeat, T.H. Beaver, G.L. Westrich and N.L. Schrader,Intraperitoneal injection of zymosan in mice induces pain, inflammation and the synthesis of peptidoleukotrienes and prostaglandin E 2 , Prostaglandins30, 769–789 (1985).
T.J. Williams,Prostaglandin E 2 ,prostaglandin I 2 and the vascular changes of inflammation, Br. J. Pharm.65, 517–524 (1979).
M. Hamberg, J. Svensson and B. Samuelsson,Thromboxanes: a new group of biologically active compounds derived from prostaglandin endoperoxydes, Proc. Natl. Acad. Sci. USA72, 2994–2998 (1975).
S. Moncada, R.J. Gryglewski, S. Bunting and J. R. Vane,An enzyme isolated from arteries transforms prostaglandin endoperoxydes to an unstable substance that inhibits platelet aggregation, Nature263, 663–665 (1976).
A.W. Ford-Hutchinson, M.A. Bray, M.V. Doing, M.E. Shipley and M.J.H. Smith,Leukotriene B 4 , a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes, Nature (London)286, 264–265 (1980).
R.M.J. Palmer, R.J. Stepney, G.A. Higgs and K.E. Eakins,Chemokinetic activity of arachidonic lipoxygenase products on leukocytes of different species, Prostaglandins20, 411–418 (1980).
P.A. Simmons, J.A. Salmon and S. Moncada,The release of leukotriene B 4 during experimental inflammation, Biochem. Pharmac.32, 1353–1359 (1983).
P.M. Brooks, D. Burton and M. J. Forest,Crystal-induced inflammation in the rat subcutaneous air-pouch, Br. J. Pharmac.90, 413–419 (1987).
R.M. Fauve, H. Jusforgues and B. Hevin,Maintenance of granuloma macrophages in serum-free medium. J. immunol. Methods64, 345–351 (1983).
S.B. Tucker, R.V. Pierre and R.E. Jordan,Rapid identification of monocytes in a mixed mononuclear cell preparation, J. Immunol. Methods14, 267–269 (1977).
H. Sors, P. Pradelles and F. Dray,Analytical methods for thromboxane B 2 measurement and validation of radioimmunoassay by gas liquid chromatography-mass spectrometry, Prostaglandins16, 277–280 (1978).
J.A. Salmon, P.M. Simmons and R.M.J. Palmer,A radioimmunoassay for leukotriene B 4 , Prostaglandins24, 225–235 (1982).
M. Bradford,A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem.72, 248–254 (1976).
G. Litwack,Photometric determination of Lysozyme activity, Proc. Soc. Exp. Biol. Med.89, 401–405 (1955).
F. Hérodin, P. Calvas, D. Dormont et R.M. Fauve,Inflammation sous-cutané induite chez la souris par le polyacrylamide, Ann. Immunol. (Inst. Pasteur)134C, 401–410 (1983).
M. A. Bray, F. M. Cunningham, A. W. Ford-Hutchinson and M. J. H. Smith,Leukotriene B 4 :a mediator of vascular permeability. Br. J. Pharm.72, 483–486 (1981).
C.V. Wedmore and T.J. Williams,Control of vascular permeability by polymorphonuclear leukocytes in inflammation, Nature289, 646–650 (1981).
G.A. Higgs, S. Moncada, J.A. Salmon and K. Seager,The source of thromboxane and prostaglandins in experimental inflammation, Br. J. Pharmac79, 863–868 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Errasfa, M., Russo-Marie, F. Characterization of a polyacrylamide gel-induced granuloma in mice: Involvement of arachidonate metabolites. Agents and Actions 24, 123–129 (1988). https://doi.org/10.1007/BF01968090
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01968090