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Alkaline phosphatase: A marker enzyme for brush border membrane

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Zusammenfassung

Mit Hilfe einer Ultramikrotechnik konnte an isolierten gewundenen und geraden Hauptstücken der Rattenniere gezeigt werden, dass die alkalische Phosphatase nicht nur in der Bürstensaum-, sondern auch in der basalen Labyrinthmembran lokalisiert ist. Das Enzym ist infolgedessen kein geeignetes Leit-Enzym zur Erkennung des Bürstensaumes aus einem Plasmamembrangemisch. Es wird vorgeschlagen, das Fehlen der Na K ATPase-Aktivität als Mass für die Reinheit der Bürstensaumfraktion zu benutzen.

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References

  1. E. Reale, Expl. Cell Res.26, 210 (1962).

    Article  CAS  Google Scholar 

  2. S. Goldfischer, E. Essner andA. B. Novikoff, J. Histochem. Cytochem.12, 72 (1964).

    Article  CAS  PubMed  Google Scholar 

  3. E. Mölbert, F. Duspiva andO. Deimling, J. biophys. biochem. Cytol.7, 387 (1960a).

    Article  PubMed Central  Google Scholar 

  4. E. Mölbert, F. Duspiva, O. v. Deimling, Histochemie2, 5 (1960b).

    Article  Google Scholar 

  5. E. Reale andL.Luciano, Histochemie8, 302 (1967).

    Article  CAS  PubMed  Google Scholar 

  6. O. H. Lowry, N. R. Roberts, M. L. Wu, W. S. Hixon andE. J. Crawford, J. biol. Chem.207, 19 (1954).

    Article  CAS  PubMed  Google Scholar 

  7. J. Rhodin, Int. Rev. Cytol.7, 485 (1958).

    Article  Google Scholar 

  8. U. Schmidt, U. C. Dubach, I. Bieder andB. Funk, Pflügers Arch. ges. Physiol.330, 265 (1972).

    Article  Google Scholar 

  9. D. R. Harkness, Arch. Biochem. Biophys.126, 513 (1968).

    Article  CAS  PubMed  Google Scholar 

  10. U. Schmidt andU. C. Dubach, Ergebn. inn. Med. Kinderheilk.32, 83 (1971).

    Google Scholar 

  11. U. Schmidt andU. C. Dubach, Progr. Histochem. Cytochem.2, 185 (1971).

    Article  Google Scholar 

  12. H. Sitte: InSekretion und Exkretion, (Ed.K. E. Wolfarth-Bottermann; Springer Verlag Berlin, Heidelberg, New York (1965), p. 343.

    Google Scholar 

  13. D. B. Roodyn, inThe Biological Basis in Medicine (EdsE. E. Bittar andN. Bittar, Academic Press, London, New York 1969), Vol. 1, p. 123.

    Google Scholar 

  14. Torhorst, H. P. Rohr andF. Gloor, Verh. dt. Ges. Path.53, 391 (1969).

    CAS  Google Scholar 

  15. M. M. Nachlas, W. Prinn andA. M. Seligman, J. biophys. biochem. Cytol.2, 487 (1956).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. R. Kinne, J. E. Schnitz andE. Kinne-Saffran, Pflügers Arch. ges. Physiol.329, 191 (1971).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Enzyme Laboratory of the Medical Policinic, University of Basel, Hebelstrasse 1, CH-4056, Basel, Switzerland

    U. Schmidt, U. C. Dubach, I. Bieder & B. Funk

  2. Institute of Pathology, University of Tübingen, Germany

    U. Schmidt, U. C. Dubach, I. Bieder & B. Funk

Authors
  1. U. Schmidt
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  2. U. C. Dubach
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  3. I. Bieder
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  4. B. Funk
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Additional information

Herrn Prof. Dr. med. Dr. h. c. Otto Gsell zum 70. Geburtstag.

Grants of SNF Nr. 3.207.69 and DFG.

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Schmidt, U., Dubach, U.C., Bieder, I. et al. Alkaline phosphatase: A marker enzyme for brush border membrane. Experientia 28, 385–386 (1972). https://doi.org/10.1007/BF02008289

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