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A simplified method for reading hemagglutinations on a flat-bottom microtitration plate in the mouse H-2 assay

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Summary

Kaliss' method for determining hemagglutination titer of mouse alloantiserum was modified to a simpler form. It became possible to read the hemagglutination directly on a flat-bottom microtitration plate using an inverted type microscope. Sensitivity of the modified method was almost the same as that of the original one. This method seems to be useful especially for the larger scale assay of H-2 specificities.

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References

  1. J. H. Stimpfling, Transplant. Bull.27, 109 (1961).

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  2. N. Kaliss, Transplantation15, 251 (1973).

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  3. F. Dacary and P. Rubinstein, Transplantation15, 630 (1973).

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Authors and Affiliations

  1. The National Institute of Genetics, Shizuoka-ken, 411, Mishima, Japan

    K. Moriwaki, T. Aotsuka & T. Shiroishi

Authors
  1. K. Moriwaki
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  2. T. Aotsuka
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  3. T. Shiroishi
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Additional information

Contribution from the National Institute of Genetics. Supported in part by grants-in-aid (No. 111510, 111504, 112105) from the Ministry of Education, Culture and Science, Japan.

Acknowledgments. The excellent technical assistance provided by Miss M. Kuranou, Mr K. Sakakibara and Miss M. Tsuyuki is gratefully acknowledged.

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Moriwaki, K., Aotsuka, T. & Shiroishi, T. A simplified method for reading hemagglutinations on a flat-bottom microtitration plate in the mouse H-2 assay. Experientia 33, 673–674 (1977). https://doi.org/10.1007/BF01946568

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  • DOI: https://doi.org/10.1007/BF01946568

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