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Satellite DNA (II) from sea urchin (Lytechinus variegatus) sperm

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Summary

When DNA isolated from freshly collected sperm of sea urchin (Lytechinus variegatus) is centrifuged to equilibrium in CsCl, 2 heavy satellite bands appear beside the main band DNA. Satellite DNA (II) appears in between the main band DNA (ϱ=1.695 g/cm3) and the rDNA satellite (ϱ=1.722 g/cm3). Satellite DNA (II) has a buoyant density 1.710 g/cm3, corresponding to 50% GC content. It is speculated that the satellite DNA (II), which appears to be of high mol.wt, might contain the sequences complementary to histone mRNA.

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Literatur

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Authors and Affiliations

  1. Postgraduate Department of Zoology, Patna University, 800005, Patna, Bihar, India

    N. K. Mishra

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  1. N. K. Mishra
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Additional information

Acknowledgment. This work was carried out in the laboratory of Dr D. W. Stafford, Department of Zoology, University of North Carolina, Chapel Hill, N.C., USA. I thank him for his help and cooperation.

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Mishra, N.K. Satellite DNA (II) from sea urchin (Lytechinus variegatus) sperm. Experientia 34, 846–848 (1978). https://doi.org/10.1007/BF01939657

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  • DOI: https://doi.org/10.1007/BF01939657

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