Summary
Apolipoprotein A-I isolated from human plasma high density lipoproteins were studied for its possible association with125I-labeled A-II. Using immunoprecipitation technique, we found that A-II, but not C-II and C-III, associated with A-I. The association was completed within 30 min and was temperature dependent.
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References
A. L. Wu and H. G. Windmuller, J. biol. Chem.254, 7316 (1979).
G. Schonfeld, E. Bell and D. H. Alpers, J. clin. Invest.61, 1539 (1978).
J. c. Osborne and H. B. Brewer, Adv. Protein Chem.31, 253 (1977).
J. B. Swaney and K. O'Brien, J. biol. Chem.253, 7069 (1978).
W. L. Stone and J. A. Reynolds, J. biol. Chem.250, 8045 (1975).
L. B. Vitello and A. M. Scanu, J. biol. Chem.251 1131 (1976).
M. Rosseneu, V. Blaton, R. Vercaemst, F. Soetewey and H. Peeters, Eur. J. Biochem.74, 83 (1977).
J. A. Reynolds and R. H. Simon, J. biol. Chem.249, 3937 (1974).
S. J. T. Mao and B. A. Kottke, Biochim. biophys. Acta260, 447 (1980).
S. I. Barr, B. A. Kottke, J. Y. Chang and S. J. T. Mao, Biochim. biophys. Acta663, 491 (1981).
S. J. T. Mao, J. P. Miller, A. M. Gotto and J. T. Sparrow, J. biol. Chem.255, 3448 (1980).
S. J. T. Mao, A. M. Gotto and R. L. Jackson, Biochemistry14, 4127 (1975).
W. P. Castelli, J. T. Doyle, T. Gordon, C. G. Hames, M. C. Hjortland, S. B. Hulley, A. Kagan and W. J. Zukel, Circulation55, 767 (1977).
J. B. Swaney and K. J. O'Brien, Biochem. biophys. Res. Commun.71, 636 (1976).
E. J. Schaefer, L. L. Jenkins and H. B. Brewer, Biochem. biophys. Res. Commun.80, 405 (1978).
S. I. Barr, B. a. Kottke and S. J. T. Mao, Am. J. clin. Nutr.34, 191 (1981).
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Acknowledgment. The authors gratefully acknowledge the assistance of Miss Susan Woychik in the preparation of the manuscript and Mrs Kay Kluge for the preparation of apoproteins. This work was supported in part by NIH grants HL 23465 and HL-27114 and by the Minnesota Heart Association grant MHA-47. S.J.T. Mao is an awardee of the Research Career Development Award grant HL-00848.
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Briones, E.R., Mao, S.J.T. Association between apolipoproteins A-I and A-II as evidenced by immunochemical approach. Experientia 38, 908–910 (1982). https://doi.org/10.1007/BF01953647
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DOI: https://doi.org/10.1007/BF01953647