Abstract
In crustaceans, all the steps in the assimilation of food take place in the hepatopancreas. To facilitate the study of this organ, a method for the dissociation of cell types was developed. The hepatopancreas of the prawnPalaemon serratus was mechanically dissociated and the cells separated by Percoll density-gradient centrifugation. The E and R cells had similar densities of around 1.05 g/ml. The F cells were separated into two distinct fractions with densities of 1.075 and 1.082 g/ml. The B cells sedimented at a density of 1.12 g/ml. The ratio between the two populations of F cells was found to vary during the intermolt cycle while B cells disappeared after the molt. When the density gradient fractions were incubated with3H-leucine, incorporation was highest in the F cell fractions. Measurements of α-amylase activity, indicated that the two populations of F cells may be derived from the same cell type.
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Literatur
Davis, L. E., and Burnett, A. L., Devl Biol.10 (1964) 122.
Jacobs, W., Z. Zellforsch.8 (1928) 1.
Hirsch, G. C., and Jacobs, W., Z. vergl. Physiol.A12 (1930) 524.
Al-Mohanna, S. Y., Nott, J. A., and Lane, D. J. W., Crustaceana48 (1985) 260.
Al-Mohanna, S. Y., and Nott, J. A., J. mar. Biol. Ass. U.K.67 (1987) 803.
Gibson, R., and Barker, P. L., Oceanogr. mar. Biol. A. Rev.17 (1979) 285.
Dall, W., and Moriarty, D. J. W., in: Biology of Crustacea, vol. 5, p. 215. Academic Press, New York 1983.
Stanier, J. E., Woodhouse, M. A., and Griffin, R. L., Crustaceana14 (1968) 56.
Loizzi, R. F., Z. Zellforsch.113 (1971) 420.
Barker, P. L., and Gibson, R., J. exp. mar. Biol. Ecol.26 (1977) 297.
Barker, P. L., and Gibson, R., J. exp. mar. Biol. Ecol.32 (1978) 177.
Al-Mohanna, S. Y., and Nott, J. A., Mar. Biol.95 (1987) 129.
Papathanassiou, E., and King, P. E., Comp. Biochem. Physiol.77 A (1984) 243.
Ogura, K., Annot. Zool. Japon32 (1959) 597.
Ahearn, G. A., Monckton, E. A., Henry, A. E., and Botfield, M. C., Am. J. Physiol.244 (1983) R150.
Bunt, A. T., Crustaceana15 (1968) 282.
Malcoste, R., van Wormhoudt, A., and Bellon, C., C. Acad. Sci., Paris296 (1983) 597.
Hopkins, S. P., and Nott, J. A., J. mar. Biol. Ass. U. K.60 (1980) 891.
Al-Mohanna, S. Y., Nott, J. A., and Lane, D. J. W., J. mar. Biol. Ass. U. K.65 (1985) 901.
Al-Mohanna, S. Y., and Nott, J. A., J. mar. Biol. Ass. U.K.66 (1986) 403.
Devillez, E. J., and Fyler, D. J., Can. J. Zool.64 (1985) 81.
Drach, P., and Tchernigotvzeff, C., Vie Milieu18 (1967) 595.
Parry, G., J. exp. Biol.31 (1964) 601.
Bernfeld, P., Meth. Enzymol.1 (1955) 149.
Richard, P., Thèse 3ème cycle, Marseille 1982.
Loret, S., van de Goor, N., and Devos, D., Oceanis15 (1989) 419.
Ellender, R. D., Middelbrooks, B. L., and MacGuire, S. L., Hawaï Congress 1988.
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Toullec, J.Y., Chikhi, M. & van Wormhoudt, A. In vitro protein synthesis and α amylase activity in F cells from hepatopancreas ofPalaemon serratus (Crustacea; Decapoda). Experientia 48, 272–277 (1992). https://doi.org/10.1007/BF01930474
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DOI: https://doi.org/10.1007/BF01930474