Abstract
The administration of 200 nM K252a to HuH7 suppressed the proliferation of the cells almost completely. The uptake of [3H]thymidine was inhibited, and flow cytometry revealed only one peak at 2C on day 3 after treatment with 100 nM K252a. The expression of proto-oncogene c-myc was not reduced. Despite the blockage at G1, both the size of the cells and the amount of cell protein had increased by 4 times by day 3 after treatment with K252a, while the cells secreted albumin and α-fetoprotein into the medium as usual. These results show that K252a can increase the cell size of HuH7 without losing its function by blocking the cell cycle at G1 phase.
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Literature
Grisham, J. W., Cancer Res.22 (1962) 842.
Nakamura, T., Nawa, K., and Ichihara, A., biochem. biophys. Res. Commun.122 (1984) 1450.
Kase, H., Iwahashi, K., and Matsuda, Y., J. Antibiotics39 (1986) 1059.
Kase, H., Iwahashi, K., Nakanishi, S., Matsuda, Y., Yamada, K., Takahashi, M., Murakata, C., Sato, A., and Kaneko, M., Biochem. biophys. Res. Commun.142 (1987) 436.
Gschwendt, M., Leibersperger, H., and Marks, F., Biochem. biophys. Res. Commun.164 (1989) 974.
Nakanishi, S., Yamada, K., Kase, H., Nakamura, S., and Nonomura, Y., J. biol. Chem.263 (1988) 6215.
Hashimoto, Y., Nakayama, T., Teramoto, T., Kato, H., Watanabe, T., Kinoshita, M., Tsukamoto, K., Tokunaga, K., Kurokawa, K., Nakanishi, S., and Nonomura, Y., Biochem. biophys. Res. Commun.181 (1991) 423.
Usui, T., Yoshida, M., Abe, K., Osada, H., Isono, K., and Beppu, T., J. Cell Biol.115 (1991) 1275.
Nakabayashi, H., Taketa, K., Miyano, K., Yamane, T., and Sato, J., Cancer Res.42 (1982) 3858.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem.193 (1951) 265.
Yoshida, M., and Beppu, T., Exp. Cell Res.177 (1988) 122.
Yoshida, M., Nishikawa, M., Nishi, K., Abe, K., Horinouchi, S., and Beppu, T., Exp. Cell. Res.187 (1990) 150.
Chomcynsky, P., and Sacci, N., Analyt. Biochem.162 (1987) 156.
Kaneko, Y., Shibuya, M., Nakayama, T., Hayashida, N., Toda, G., Endo, Y., Oka, H., and Oda, T., Jap. J. Cancer Res.76 (1985) 1136.
Marcu, K. B., Bossone, S. A., and Patel, A. J., A. Rev. Biochem.61 (1992) 809.
Kelly, K., Cochran, B. H., Stiles, C. D., and Leder, P., Cell35 (1983) 603.
Makino, R., Hayashi, K., and Sugimura, T., Nature310 (1984) 697.
Chafouleas, J. G., Lagace, L., Bolton, W. E., Boyd III, A. E., and Means, A. R., Cell36 (1984) 73.
Nishikawa, Y., Yamamoto, Y., Kaji, K., and Matsui, H., Biochem. biophys. Res. Commun.97 (1980) 1296.
Candido, E. P. M., Reeves, R., and Davie, J. R., Cell14 (1978) 105.
Abe, K., Yoshida, M., Usui, T., Horinouchi, S., and Beppu, T., Exp. Cell Res.192 (1991) 122.
Ohta, Y., Ohba, T., Fukunaga, K., and Miyamoto, E., J. biol. Chem.263 (1988) 11540.
Ohta, Y., Ohba, T., and Miyamoto, E., Proc. natl Acad. Sci. USA87 (1990) 5341.
Yamamoto, H., Fukunaga, K., Goto, S., Tanaka, E., and Miyamoto, E., J. Neurochem.44 (1985) 759.
Gotoh, Y., Nishida, E., Matsuda, S., Shiina, N., Kosako, H., Shiokawa, K., Akiyama, T., Ohta, K., and Sakai, H., Nature349 (1991) 251.
Ohmi, K., Yamashita, S., and Nonomura, Y., Biochem. biophys. Res. Commun.173 (1990) 976.
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Nakayama, T., Hashimoto, Y., Kaneko, Y. et al. K252a: A new blocker of the cell-cycle at G1 phase in a human hepatoma cell line. Experientia 49, 876–880 (1993). https://doi.org/10.1007/BF01952601
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DOI: https://doi.org/10.1007/BF01952601