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Complementation analysis with new genetic markers in Phaffia rhodozyma

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Abstract

Isolation and characterization of auxotrophic mutants from wild-type and astaxanthin mutant strains of Phaffia rhodozyma is described. Differences in survival were observed when u.v. irradiation of P. rhodozyma wild-type and astaxanthin mutant strains were incubated in the dark or exposed to photoreactivating light. Ultra-violet mutagenesis was not effective to produce auxotrophic mutants in this yeast. Auxotrophic mutants were obtained with high efficiency through a nystatin enrichment procedure after a N-methyl-N′-nitro-N-nitrosoguanidine (NTG) mutagenic treatment with a 0.12% survivor level. Stringent mutagenetic conditions were needed to obtain P. rhodozyma auxotrophs. The most frequent mutants were ade- and met- in a rather narrow auxotroph spectrum. These results may be associated with a possible diploid condition of this yeast. The high number of adenine auxotrophs obtained in relation to other auxotrophic mutants suggests the possibility of some degree of heterozygosity in the wild-type strain UCD 67-385.

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Authors and Affiliations

  1. Laboratorio de Genética, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile

    P. Retamales, R. León, C. Martínez, G. Hermosilla, G. Pincheira & V. Cifuentes

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  1. P. Retamales
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  2. R. León
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  3. C. Martínez
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  4. G. Hermosilla
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  5. G. Pincheira
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  6. V. Cifuentes
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Retamales, P., León, R., Martínez, C. et al. Complementation analysis with new genetic markers in Phaffia rhodozyma. Antonie Van Leeuwenhoek 73, 229–236 (1998). https://doi.org/10.1023/A:1000828220033

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