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Size analysis of poly(3-hydroxybutyric acid) granules produced in recombinant Escherichia coli

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Summary

Size distributions of PHB granules synthesized in recombinant Escherichia coli are determined by photosedimentation. Mean granule Stokes diameters are in the range 1.13–1.25 μm, which is larger than reported values for wild type microorganisms. Treatment with 1.5% hypochlorite and mild centrifugation did not affect granule size distribution. Treatment with 10% hypochlorite led to a significant reduction in mean diameter and total PHB.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Adelaide, 5005, Adelaide, S.A., Australia

    Anton P. J. Middelberg, Jennifer Martin & David R. G. Williams

  2. Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technologyrea Advanced Institute of Science and Technologyrea Advanced Institute of Science and Technology, 305-701, Taejon, Korea

    Sang Yup Lee & Ho Nam Chang

Authors
  1. Anton P. J. Middelberg
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  2. Sang Yup Lee
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  3. Jennifer Martin
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  4. David R. G. Williams
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  5. Ho Nam Chang
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Middelberg, A.P.J., Lee, S.Y., Martin, J. et al. Size analysis of poly(3-hydroxybutyric acid) granules produced in recombinant Escherichia coli . Biotechnol Lett 17, 205–210 (1995). https://doi.org/10.1007/BF00127989

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  • DOI: https://doi.org/10.1007/BF00127989

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