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Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies

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Abstract

The purification of a DNA vaccine against rabies by hydrophobic interaction chromatography (HIC) using a Sepharose gel derivatised with 1,4-butanediol diglycidyl ether was scaled up 60 times. The purification profile was not affected by increased loadings (up to 15 mg DNA) and a product with a consistent quality was obtained. Fourteen mg of plasmid with an HPLC purity of 100% were obtained in one run, corresponding to a 95% yield.

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Authors and Affiliations

  1. Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001, Lisboa, Portugal

    M.M. Diogo, S. Ribeiro, G.A. Monteiro & D.M.F. Prazeres

  2. Departamento de Química, Universidade da Beira Interior, 6200, Covilhã, Portugal

    J.A. Queiroz

  3. Laboratoire des Lyssavirus, Institute Pasteur, Paris, France

    P. Perrin & N. Tordo

Authors
  1. M.M. Diogo
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  2. S. Ribeiro
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  3. J.A. Queiroz
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  4. G.A. Monteiro
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  5. P. Perrin
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  6. N. Tordo
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  7. D.M.F. Prazeres
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Diogo, M., Ribeiro, S., Queiroz, J. et al. Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies. Biotechnology Letters 22, 1397–1400 (2000). https://doi.org/10.1023/A:1005665003375

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  • DOI: https://doi.org/10.1023/A:1005665003375

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