Summary
Relatively high levels of nutrients and low levels of waste products were maintained in cultures of hybridoma cells by dialysis against growth medium supplemented with fetal calf serum (FCS). This resulted in a greater than ten fold increase in cell density and monoclonal antibody titre compared to conventional batch cultures. Exclusion of FCS from the dialysing growth medium resulted in cell death, indicating that a low molecular weight serum component was necessary for growth. This study indicates that nutrient depletion limits hybridoma cell growth in conventional batch culture and also provides a simple and economical means of producing relatively high titre monoclonal antibody in vitro.
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References
Kohler, G., and Milstein, C. (1975) Nature 256, 56–64.
Edwards, P.A.W. (1981) Biochem. J. 200, 1–10.
Goding, J.W. (1980) J. Immunol. Methods 39, 285–308.
Voller, A., Bidwell, D.E., and Bartlett, A. (1976) Bull. World Health Organ. 53, 55–65.
Murakami, H., Masui, H., Sato, G.H., Sueoka, N., Chown, T.P., and Kano-Sueoka, T. (1982) Proc. Natl. Acad. Sci. 79, 1158–1162.
Bundle, D.R., Gidney, M.A.J., Kassam, N., and Rahman, A.F.R. (1982) J. Immunol. 129, 678–682.
Calabresi, P., McCarthy, K.L., Dexter, D.L., Cummings, F.J., and Rotman, B. (1981) Proc. AACR and ASCO abstr. 1200, 302.
Jarvis, A.P., Spriggs, T.A., Chipura, W.R., Sullivan, M., and Koch, G.A. (1982) In Vitro 18, (TCA meeting abst.) 276.
Lim, F., and Sun, A.M. (1980) Science 210, 908–910.
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A publication of the University of Calgary Immobilized Cell Biotechnology Group.
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Adamson, S.R., Fitzpatrick, S.L., Behie, L.A. et al. In vitro production of high titre monoclonal antibody by hybridoma cells in dialysis culture. Biotechnol Lett 5, 573–578 (1983). https://doi.org/10.1007/BF00130835
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DOI: https://doi.org/10.1007/BF00130835