Summary
A simple aspartase assay was developed. Aspartase fromEscherichia coli Crooks strain was adsorbed to β-naphthyl cotton cloth by hydrophobic interaction. The adsorbed enzyme did not desorb in 1 M ammonium fumarate. The adsorbed enzyme exhibited the same pH vs. activity curve as free enzyme and had a half life of approx. 40 weeks. A column packed with the adsorbed aspartase showed 100% conversion of 1 M ammonium fumarate at a space velocity of approx. 2.
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Yamazaki, H., Sharma, S. & Joshi, S. Adsorption of aspartase onto hydrophobic cloth for conversion of ammonium fumarate. Biotechnol Lett 8, 107–110 (1986). https://doi.org/10.1007/BF01048466
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DOI: https://doi.org/10.1007/BF01048466