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Iron compounds at high concentrations enable hybridoma growth in A protein-free medium

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Summary

Soluble iron salts at very high concentrations (50 μM to 5 mM), substituting for the only indispensable protein transferrin, enable hybridoma growth and monoclonal antibody production in a chemically defined protein-free medium.

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Authors and Affiliations

  1. Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Vídeňská 1083, CS-14220, Praha 4, Czechoslovakia

    Jan Kovář & František Franěk

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  1. Jan Kovář
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  2. František Franěk
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Kovář, J., Franěk, F. Iron compounds at high concentrations enable hybridoma growth in A protein-free medium. Biotechnol Lett 9, 259–264 (1987). https://doi.org/10.1007/BF01027160

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  • DOI: https://doi.org/10.1007/BF01027160

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