The effects of exogenous and endogenous granulocyte colony-stimulating factor (G-CSF) on invasion by cancer cells were studied, using lung cancer cell lines that produce G-CSF (NCI-H157) and lines that do not (PC-9 and NCI-H23). The invasive capacity of NCI-H157 cells was 26- to 27-fold higher than that of PC-9 and NCI-H23 cells. The invasiveness of PC-9 cells was stimulated by exogenous G-CSF, while that of NCI-H157 cells was not. Antibodies against G-CSF blocked the stimulation of PC-9 cell invasiveness by exogenous G-CSF. Anti G-CSF antibodies also inhibited invasion by NCI-H157 cells in the absence of exogenous G-CSF. These results suggest that endogenous and exogenous G-CSF both stimulate invasion by lung cancer cells.
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References
LiottaRao CN and Barsky SH, 1983, Tumor invasion and the extracellular matrix, Lab Invest, 49, 636–49.
Wei W and Heppner GH, 1988, Tumor infiltrating lymphocytes of spontaneous versus transplanted mouse mammary tumors. Cancer Immunol Immunother, 26, 257–62.
Rice GE, Gimbrone MA and Bevilacqua MP, 1988, Tumor cell-endothelial interaction. Am J Pathol, 133, 204–11.
Breillout F, Antoine E, Lascaus V, Rolland Y and Poupon MF, 1989, Promotion of micrometastasis proliferation in a rat rhabdomyosarcoma model by epidermal growth factor. J Natl Cancer Inst, 81, 702–5.
Li X, Nagayasu H, Hamada J, Hosokawa M and Takeichi N, 1993, Enhancement of tumorigenicity and invasion capacity of rat mammary adenocarcinoma cell by epidermal growth factor and transforming growth factor-β. Jpn J Cancer Res, 84, 1145–9.
Kacinski BM, Scata KA, Carter D, et al. 1991, FMS (CSF-1 receptor) and CSF-1 transcripts and protein are expressed by human breast carcinoma in vivo and in vitro. Oncogene, 6, 941–52.
Filderman AB, Bruckner A, Kacinski BM, Deng N and Remold HG, 1992, Macrophage colonystimulating factor (CSF-1) enhances invasiveness in CSF-1 receptor-positive carcinoma cell lines. Cancer Res, 32, 3661–6.
Dabbous MK, North SM, Haney L and Nicolson GL, 1988, Macrophage and lymphocyte potentiation of syngeneic tumor cell and host fibroblast collagenolytic activity in rats. Cancer Res, 48, 6832–6.
Pedersen PH, Ness GO, Engebraaten O, Bjerkvig R, Liliehaug JR and Laerum OD, 1994, Heterogenous response to the growth factors [EGF, PDGF (bb), TGF-α, bFGF, IL-2] on glioma spheroid growth, migration and invasion. In J Cancer, 56, 255–61.
Harmenberg J, Hoglund M and Hellstrom-Lindberg E, 1994, G- and GM-CSF in oncology and oncological haematology. Eur J Haematol, 52, 1–28.
Motoji T, Watanabe M, Uzumaki H, et al. 1991, Granulocyte colony-stimulating factor (G-CSF) receptors on acute myeloblastic leukaemia cells and their relationship with the proliferative response to G-CSF in clonogenic assay. Br J Haematol, 77, 54–9.
Avoloa BR, Gasson JC, Hedvat C, et al. 1990, Human granulocyte colony-stimulating factor biologic activities and receptor characterization on hematopoietic cells and small cell lung cancer cell lines. Blood, 75, 851–7.
Bussolino F, Wang JM, Defilippi P, et al. 1989, Granulocyte and granulocyte-macrophage-colony stimulating factors induce human endothelial cells to migrate and proliferate. Nature, 337, 471–3.
Berdel WE, Danhouser-Riedl S, Steinhauser G and Winton EF, 1989, Various human hematopoietic growth factors (interleukin 3, GM-CSF, G-CSF) stimulate clonal growth of nonhematopoietic tumor cells. Blood, 73, 80–3.
Uzumaki H, Okabe T, Sasaki N, et al. 1989, Identification and characterization of receptors for granulocyte colony-stimulating factor on human placenta and trophoblastic cells. Proc Natl Acad Sci USA, 86, 9323–6.
Motojima H, Kobayashi T, Shimane M, Kamachi S and Fukushima M, 1989, Quantitative enzyme immunoassay for human granulocyte colony stimulating factor (G-CSF). J Immunol Method, 118, 187–92.
Hendrix MJC, Seftor EA, Seftor REB and Fidler IJ, 1987, A simple quantitative assay for studying the invasive potential of high and low human metastatic variants. Cancer Lett, 38, 137–47.
Albini A, Iwamoto Y, Kleinman HK, et al. 1987, A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res, 47, 3239–45.
Mosmann T, 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods, 65, 55–63.
Nakanishi Y, Mulshine JL, Kasprzyk PG, et al, 1988, Insulin-like growth factor-1 can mediate autocrine proliferation of human small cell lung cancer cell lines in vitro. J Clin Invest, 82, 354–9.
Rapoport AP, Abboud CN and Dipersio JF, 1992, Granulocyte macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF): receptor biology, signal transduction and neutrophil activation. Blood, 6, 42–57.
Young MRI, Lozano Y, Coogan M, Wright MA, Young ME and Bagash JM, 1992, Stimulation of the metastatic properties of Lewis lung-carcinoma cells by autologous granulocyte-macrophage colony stimulating-factor. Int J Cancer, 50, 628–34.
Young MRI, Lozano Y, Djordjevic A, et al. 1993, Granulocyte-macrophage colony-stimulating factor stimulates the metastatic properties of Lewis lung carcinoma cells through a protein kinase. A signaltransduction pathway. Int J Cancer, 53 667–71.
Kobayashi H, Gotoh J, Fujie M and Terao T, 1994, Characterization of the cellular binding site for the urinary trypsin inhibitor. J Biol Chem, 269, 20642–7.
Pietsch T, Kyas U, Steffens U, et al. 1992, Effect of human stem cell factor (c-kit ligand) on proliferation of mycloid leukemia cells: heterogeneity in response and synergy with other hematopoietic growth factors. Blood, 80, 1199–206.
Singh RK, Gutman M, Radinsky R, Bucana CD and Fidler IJ, 1994, Expression of interleukin 8 correlates with the metastatic potential of human melanoma cells in nude mouse. Cancer Res, 54, 3242–47.
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Pei, XH., Nakanishi, Y., Takayama, K. et al. Granulocyte-colony stimulating factor promotes invasion by human lung cancer cell lines in vitro . Clin Exp Metast 14, 351–357 (1996). https://doi.org/10.1007/BF00123394
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DOI: https://doi.org/10.1007/BF00123394