Abstract
A total of 1226 sera from 1055 patients with respiratory tract infections were tested. IgM antibodies were detected by an antibody-capture enzyme-immunoassay (Mp TEST, Diatech Diagnostica Ltd, Israel). Acute infection with IgM antibodies to Mycoplasma pneumoniae was detected in 211 patients.
Presence of IgM was closely associated to some or all pneumonia-related symptoms. Eighty-one of IgM-positive patients treated with tetracycline or erythromycin responded positively. Of the 211 patients, 63 (30%) had low levels and 23 (11%) had moderate levels of IgM antibodies already in the first serum sample. In these 86 patients (41%) the complement fixation (CF) test was negative or very low positive. Thus in these cases, the CF test would have missed the early diagnosis in the first serum samples.
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References
Barker C.E., Siblis M. and Wreghitt T.G. (1991): Evaluation of Serodia Myco 11 particle agglutination test for detection of Mycoplasma pneumoniae antibody: comparison with u-capture ELISA and indirect immunofluorescence -J. Clin. Pathol. 43: 163–165.
Cassel G.H. and Cole B.C. (1981): Mycoplasma agents of human disease -N. Eng. J. Med. 304: 80–89.
Echevarria J.M., Leon P., Balfagon P., Lopez J.A. and Fernandez M.V. (1990): Diagnosis of Mycoplasma pneumoniae infection by microparticle agglutination and antibody capture enzyme-immunoassay -Eur. J. Clin. Microbiol. Inf. Dis. 9: 217–220.
Finnegan O.C., Fowles S.J. and White R.J. (1981): Radiographic appearance of Mycoplasma pneumoniae -Thorax 36: 469–472.
Hirschberg L., Krook A., Pettersson C.A. and Vikersfors T. (1988): Enzyme-linked immunosorbent assay for detection of Mycoplasma pneumoniae specific immunoglobulin M. -Eur. J. Clin. Microbiol. Inf. Dis. 7: 420–423.
Levine D.P. and Lerner A.M. (1978): The clinical spectrum of Mycoplasma pneumoniae infections -Med. Clin. North Am. 72: 961–978.
Moule J.H. and Caul E.0. (1987): The specific IgM response to Mycoplasma pneumoniae infection: interpretation and application to early diagnosis -Epid. Inf. 99: 685–692.
Ponka A., Ponka T., Sarna S. and Penttinen K. (1981): Questionable specificity of lipid antigen in Mycoplasma pneumoniae CF test in patients with extrapulmonary manifestation -J. Infection 3: 332–338.
Tilton R.C., Dias F., Kidd H. and Ryan R.W. (1988): DNA probe versus culture for detection of Mycoplasma pneumoniae in clinical specimens 10: 109–112.
Vikerfors T., Bordin G., Grandien M., Hirschberg L., Brook A. and Petterson C. (1988): Detection of specific IgM antibodies for the diagnosis of Mycoplasma pneumoniae infection: A clinical evaluation -Scand. J. Inf. Dis. 20: 601–610.
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Samra, Z., Gadba, R. Diagnosis of Mycoplasma pneumoniae infection by specific IgM antibodies using a new capture-enzyme-immunoassay. Eur J Epidemiol 9, 97–99 (1993). https://doi.org/10.1007/BF00463097
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DOI: https://doi.org/10.1007/BF00463097