Summary
The endothelial cells of mammalian brain capillaries, which form the anatomical basis of the blood-brain barrier, have been investigated by immunocytochemical methods to determine the distribution of the glucose-transport protein. A monoclonal antibody raised against the intact human erythrocyte glucose-transport protein and polyclonal antibodies raised against a synthetic peptide corresponding to the C-terminal sequence of the human erythrocyte glucose-transport protein were used for immunofluorescent staining of isolated human and bovine cerebral cortex microvessels. The pattern of fluorescence with both antibodies demonstrated the antigen to the distributed throughout the plasma membrane of the capillary endothelial cells. These results provide further evidence for the homology between the human erythrocyte and brain capillary glucose-transport protein, and confirm its abundance in brain capillaries.
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Allard, W. J. &Lienhard, G. E. (1985) Monoclonal antibodies to the glucose transporter from human erythrocytes.J. biol. Chem. 260, 8668–75.
Baldwin, S. A., Baldwin, J. M. &Lienhard, G. E. (1982) Monosaccharide transporter of the human erythrocyte: characterization of an improved preparation.Biochemistry 21, 3836–42.
Baldwin, S. A., Cairns, M. T., Gardiner, R. M. &Ruggier, R. (1985). Ad-glucose sensitive cytochalasin B binding component of cerebral microvessels.J. Neurochem. 45, 650–2.
Bar, T. (1980)The Vascular System of the Cerebral Cortex. Berlin: Springer-Verlag.
Birnbaum, M. J., Haspel, H. C., &Rosen, O. M. (1986) Cloning and characterisation of a cDNA encoding the rat brain glucose transport proteins.Proc. natn. Acad. Sci. U.S.A. 83, 5784–8.
Boyle, J. M., Whetton, A. D., Dexter, T. M., Meeran, K. &Baldwin, S. A. (1985) Characterisation of monoclonal antibodies which specifically recognise the human erythrocyte glucose transport proteinEMBO J. 4(12), 3093–8.
Bradbury, M., (1979)The Concept of a Blood-Brain Barrier pp. 60–83, 137–84. Chichester: John Wiley and Sons.
Cairns, M. T., Elliot, D. A., Scudder, P. R. &Baldwin, S. A. (1984) Proteolytic and chemical dissection of the human erythrocyte glucose transporter.Biochem. J. 221, 179–88.
Davies, A., Meeran, K., Cairns, M. T. &Baldwin, S. A. (1987)J. biol. Chem. 262, 9347–52.
Dick, A. P. K. &Harik, S. I. (1986). Distribution of the glucose transporter in the mammalian brain.J. Neurochem. 46, 1406–11.
Dick, A. P. K., Harik, S. I., Klip, A. &Walker, D. M. (1984) Identification and characterization of the glucose transporter of the blood-brain barrier by cytochalasin B binding and immunological reactivity.Proc. natn. Acad. Sci. U.S.A. 81, 7233–7.
Flier, J. S., Mueckler, M., McCall, A. L. &Lodish, H. F. (1987) Distribution of glucose transporter messenger RNA transcripts in tissues of rat and man.J. Clin. Invest. 79, 657–61.
Kasanicki, M. A., Cairns, M. T., Davies, A., Gardiner, R. M. &Baldwin, S. A. (1987) Identification and characterisation of the glucose transport protein of the bovine blood-brain barrier.Biochem. J. 247, 101–8.
Mueckler, M., Caruso, C., Baldwins, S. A., Panico, M., Blench, I., Morris, H. R., Leinhard, G. E., Allard, W. J. &Lodish, H. F. (1985) Sequence and structure of a human glucose transporter.Science 229, 941–5.
Pardridge, W. M. (1983) Brain metabolism — a perspective from the blood brain barrier.Physiol. Rev. 63, 1481–535.
Timpl, R., Rhode, H., Robey, P. G., Rennard, S. I., Foidart, J. M. &Martin, G. R. (1979) Laminin: a glycoprotein from basement membranes.J. biol. Chem. 254(19), 9933–7.
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Kasanicki, M.A., Jessen, K.R., Baldwin, S.A. et al. Immunocytochemical localization of the glucose-transport protein in mammalian brain capillaries. Histochem J 21, 47–51 (1989). https://doi.org/10.1007/BF01002471
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DOI: https://doi.org/10.1007/BF01002471