Abstract
A new isotope-assisted cross-relaxation editing experiment, [1H-13C]DINE-NOESY[1H-15N]HSQC (DINE = Double INEPT Edited), is proposed. It is based on the selectiveinversion of CH/CH3 or CH2 protons in the middle of the mixing time. The experiment sortsout the spin diffusion paths according to the principal mediators, either the CH/CH3 or theCH2 protons. This is useful in the structure refinement process, as it enables proper alignmentof the aliphatic protons in the vicinity of NH protons.
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Juranic, N., Zolnai, Z. & Macura, S. Identification of spin diffusion pathways in proteins by isotope-assisted NMR cross- relaxation network editing. J Biomol NMR 9, 317–322 (1997). https://doi.org/10.1023/A:1018639211400
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DOI: https://doi.org/10.1023/A:1018639211400