Abstract
Human pluripotential stem cells (PSC) are currently the target fortransplantation attempts and genetic manipu-lation. We have thereforeinvestigated the frequency and the expansion potential of PSC's in differenttypes of blood samples. CD 34+ cells were thus obtained from human bonemarrow (BM), as well as from peripheral blood (PB) and cord blood (CB)samples. After immuno-magnetic separation the highest yields of CD 34+cells were from BM (1.08-2.25%) and CB (0.42-1.32%) while PBsamples gave much lower values. Suspension cultures of PSC's from the threesources were then set up, in the presence of combinations of haemopoieticgrowth factors. A remarkable amplification of the nucleated cell pool wasobserved reaching a maximum between 10 and 15 days of culture; earliest andmaximum expansion (up to 220-fold) was achieved when Erythropoietin (Epo)was added to the culture medium, but this resulted in reduction ofcolony-forming cells and differentiation into erythroid progenitors.Clonogenic tests for BFU-E's derived colonies showed a peak value at 5 daysof liquid culture. Further studies are advisable to establish the bestcytokine combination for a valuable ex vivo expansion, coupled withpreservation of stem cell properties.
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Eridani, S., Mazza, U., Massaro, P. et al. Cytokine effect on ex vivo expansion of haemopoietic stem cells from different human sources. Biotherapy 11, 291–296 (1998). https://doi.org/10.1023/A:1008081708054
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DOI: https://doi.org/10.1023/A:1008081708054