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Determination of D-amino acids using a D-amino acid oxidase biosensor with spectrophotometric and potentiometric detection

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Biotechnology Techniques

Abstract

An amperometric and a colorimetric biosensor to detect and quantify D-amino acids selectively has been devised using D-amino acid oxidase from Rhodotorula gracilis. The sensor is characterised by a proportional response between 0.2-3 mM and 0.1-1 mM D-alanine for the amperometric (at a working potential of 1400 mV vs Ag/AgCl) and colorimetric system, respectively.

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Authors and Affiliations

  1. Department of Structural and Functional Biology, University of Milano, via Ravasi, Varese, Italy

    Silvia Sacchi

Authors
  1. Silvia Sacchi
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  2. Loredano Pollegioni
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  3. Mirella S. Pilone
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  4. Carlo Rossetti
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Sacchi, S., Pollegioni, L., Pilone, M.S. et al. Determination of D-amino acids using a D-amino acid oxidase biosensor with spectrophotometric and potentiometric detection. Biotechnology Techniques 12, 149–153 (1998). https://doi.org/10.1023/A:1008896602544

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  • DOI: https://doi.org/10.1023/A:1008896602544

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