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Rapid preparation in high yield of pure formate dehydrogenase from Pichia angusta

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Biotechnology Techniques

Abstract

Biomimetic dye ligand chromatography and reversible ionic strength-dependent protein precipitation enabled isolation of formate dehydrogenase from the yeast Pichia angusta in purity of >95%. The enzyme has a specific NAD-linked activity of 5.7 units/mg and was obtained in stable form, yield of 88% and concentration of about 20 mg/ml. © Rapid Science Ltd. 1998

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Authors
  1. W. Neuhauser
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  2. M. Steininger
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  3. D. Haltrich
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  4. K.D. Kulbe
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  5. B. Nidetzky
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Neuhauser, W., Steininger, M., Haltrich, D. et al. Rapid preparation in high yield of pure formate dehydrogenase from Pichia angusta. Biotechnology Techniques 12, 565–568 (1998). https://doi.org/10.1023/A:1008867901427

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  • DOI: https://doi.org/10.1023/A:1008867901427

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