Abstract
Apoptosis-inducing materials were produced by digesting bovine skimmed milk with cell-free extract of Saccharomyces cereviiae at pH 4.8. An enzyme involved in production of the materials was purified from the cell-free extract by successive column chromatography. The purified enzyme was homogeneous and identified as protease B by analyzing N-terminal amino acid sequence. Characteristics features of apoptosis were observed within 5 h of digested skimmed milk treatment as documented by DNA fragmentation, expression of phosphatidylserine. The inducing factors were recovered in the soluble fraction of 92% ethanol, suggesting that the factors were hydrophilic low molecular weight substances.
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Kumar Roy, M., Watanabe, Y. & Tamai, Y. Induction of apoptosis in human leukemia (HL-60) cells by skimmed milk digested with a proteolytic enzyme purified from Saccharomyces cerevisiae. Biotechnology Techniques 13, 727–734 (1999). https://doi.org/10.1023/A:1008946616614
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DOI: https://doi.org/10.1023/A:1008946616614