Abstract
A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.
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References
Beaucage SL, Caruthers MH (1981) Tetrahedron Lett. 22: 1859-1862.
Brückner R (1992) Gene 122: 187-192.
Diderichsen B, Christiansen L (1985) FEMS Microbiol. Lett. 56: 53-60.
Fleming AB, Tangney M, Jørgensen PL, Diderichsen B, Priest FG (1995) Appl. Environ. Microbiol. 61: 3775-3780.
Jørgensen ST, Tangney M, Jørgensen PL, Diderichsen B (1998) Biotechnol. Tech. 12: 15-19.
Laemmli UK (1970) Nature 227: 680-685.
Mongkolsuk S, Duvall EJ, Lovett PS (1985) Gene 37: 83-90.
Tangney M, Brehm J, Minton N, Mitchell WJ (1998) Appl. Environ. Microbiol. 64: 1612-1619.
Tangney M, Jørgensen PL, Diderichsen B, Jørgensen ST (1995) FEMS Microbiol. Lett. 125: 107-114.
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Tangney, M., Mitchell, W.J. & Dolberg-Rasmussen, M. Development of a shuttle vector for screening of strong promoter sequences. Biotechnology Techniques 13, 141–144 (1999). https://doi.org/10.1023/A:1008983208118
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DOI: https://doi.org/10.1023/A:1008983208118