Summary
This work presents the optimization of the chemical steps involved in nylon modification with dimethyl sulphate, polyethyleneimine, glutaraldehyde and 2-diethyl aminoethylamine to obtain a weak basic anion exchange support. Activated nylon laminated membranes were utilized for aminoacylase immobilization, allowing an ionically adsorbed enzyme derivative with high activity (0.16 U/mg E·cm2) and low removed activity (<1%). Optimum immobilization conditions and kinetic parameters were also determined. This immobilized enzyme can be used in laminated enzyme membrane reactors.
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Obón, J.M., Manjón, A., Cánovas, M. et al. Anionic exchange nylon laminated membranes for enzyme immobilization. Biotechnol Tech 4, 357–362 (1990). https://doi.org/10.1007/BF00157436
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DOI: https://doi.org/10.1007/BF00157436