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Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis inEscherichia coli batch fermentations

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Biotechnology Techniques

Summary

A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation (RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity inEscherichia coli batch fermentation.

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Authors and Affiliations

  1. Instituto de Ingeniería Bioquímica (CSIC-UAB), Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain

    Valero F., Lafuente F. J. & Solà C.

  2. Institut de Biologia Fonamental i Departament de Genética i Microbiologia, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain

    Benito A., Vidal M., Cairó J. & Villaverde A.

Authors
  1. Valero F.
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  2. Lafuente F. J.
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  3. Solà C.
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  4. Benito A.
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  5. Vidal M.
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  6. Cairó J.
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  7. Villaverde A.
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Valero, F., Lafuente, F.J., Solà, C. et al. Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis inEscherichia coli batch fermentations. Biotechnol Tech 6, 213–218 (1992). https://doi.org/10.1007/BF02439346

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  • DOI: https://doi.org/10.1007/BF02439346

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