Skip to main content
Log in

Comparative methods for isolation of Volcaniella eurihalina exopolysaccharide

  • Published:
Biotechnology Techniques

Summary

The best isolation procedure to obtain the EPS of Volcaniella eurihalina was centrifugation at 36,000 x g for 60 min and precipitation with cold ethanol after tangential filtration with 100,000 D ultrafilters. Ion chromatography showed that this EPS contained glucose, rhamnose and mannose in a molar ratio of 3.2: 1.1:1, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Allen, P.M., Williams, J.M., Hart, C.A. and Saunders, J.R. (1987). J. Gen. Microbiol. 133, 1365–1370.

    Google Scholar 

  • Beech, I.B., Gaylarde, C.C., Smith, J.J. and Geesey, G.G. (1991) Appl. Microbiol. Biotechnol. 35, 65–71.

    Google Scholar 

  • Blumenkranz, N. and Asboe-Hansen, G. (1973). Anal. Biochem. 54, 484–489.

    Google Scholar 

  • Cerning, J., Bouillane, C. and Desmazeaud, M.J. (1988). Biotechnol. Letters 10 (4), 255–260.

    Google Scholar 

  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956). Anal. Chem. 28, 350–355.

    Google Scholar 

  • Fazio, S.A., Uhlinger, D. J., Parker, J.H. and White D.C. (1982). Appl. Environ. Microbiol. 43, 1151–1159.

    Google Scholar 

  • Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951). J. Biol. Chem. 193, 265–275.

    Google Scholar 

  • Meade, M.J., Tanenbaum, S.W. and Nakas, J.P. (1994). Appl. Environ. Microbiol. 60 (4), 1367–1369.

    Google Scholar 

  • Mian, F.A., Jarman, T.R. and Righelato, R.C. (1978). J.Bacteriol. 134, 418–422.

    Google Scholar 

  • Mody, B., Bindra, M. and Modi, V. (1989). Arch. Microbiol. 153, 38–42.

    Google Scholar 

  • Moraine, R.A. and Rogovin, P. (1966). Biotechnol. and Bioeng. 8, 511–524.

    Google Scholar 

  • Quesada, E., Valderrama, M.J., Bejar, V.,Ventosa, A., Gutierrez, C., Ruiz Berraquero, F. and Ramos-Cormenzana, A. (1990). Int. J. Syst. Bacteriol. 40, 261–267.

    Google Scholar 

  • Quesada, E., Bejar, V. and Calvo, C. (1993). Experientia 49 (12), 1037–1041.

    Google Scholar 

  • Read, R.R. and Costerton, J.W. (1987). Can. J. Microbiol. 33 (12), 1080–1090.

    Google Scholar 

  • Rodriguez-Valera, F., Ruiz-Berraquero, F. and Ramos-Cormenzana, A. (1981). Microb. Ecol. 7, 235–243.

    Google Scholar 

  • Rosenberg, E. (1986). CRC Critical Reviews in Biotechnology 3, 109–132.

    Google Scholar 

  • Rosenberg, E., Rubinovitz, C., Legmann, R. and Ron, E.Z. (1988). Appl. Environ. Microbiol. 54 (2), 323–326.

    Google Scholar 

  • Savidge, R.A. and Colvin, J.R. (1985). Can. J. Microbiol. 31 (11), 1019–1025.

    Google Scholar 

  • Sutherland, I.W. (1990). Biotechnology of Microbial exopolysaccharides. Cambridge University Press. Cambridge.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Quesada, E., del Moral, A. & Béjar, V. Comparative methods for isolation of Volcaniella eurihalina exopolysaccharide. Biotechnol Tech 8, 701–706 (1994). https://doi.org/10.1007/BF00151472

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00151472

Keywords

Navigation