Summary
Interleukin 2 activity is usually determined by a proliferation assay using an IL-2-dependent cell line. Tritiated thymidine incorporation during DNA synthesis is a suitable method for this purpose, but its main drawback is the use of radioactive isotopes. We describe the use of Alamar Blue, a new fluorogenic growth indicator, for the measurement of interleukin 2 activity in microtitration plates. This assay is sensitive and economical. The lower limit of detection is about 400 cells per well with an intra-assay coefficient of variation of about 5 percent.
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Harvey, M., Pagé, B. & Pagé, M. Determination of interleukin 2 activity by a new fluorometric method. Biotechnol Tech 9, 69–73 (1995). https://doi.org/10.1007/BF00153004
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DOI: https://doi.org/10.1007/BF00153004