Skip to main content
SpringerLink
Log in

Relationship between antibody productivity by activated human lymph node lymphocytes from lung cancer patients and lymphocyte subsets

  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

Regional lymph node lymphocytes from five patients with primary lung cancer were analyzed for subset composition, and exposed in vitro to the polyclonal human B cell mitogen Staphylococcus aureus Cowan I (SACI) or the murine B cell mitogen lipopolysaccharide (LPS) and then fused with mouse myeloma cells for investigation at the clonal level of their antibody (Ab) production and its statistical relation to the original subset composition. No correlation was found between the proportion of CD19+, CD23+, or CD3+ cells in the lymphocyte sample prior to its exposure to either SACI or LPS, and the Ab production efficiency, defined as the ratio of the number of Ab producing wells to the total number of proliferating wells. For lymphocytes exposed to LPS, however, a strong correlation (r = 0.931, p = 0.02) was observed between the Ab production efficiency and the ratio of CD8+ to CD3+ cells (CD8/CD3) in the original sample at least within the ranges studied (CD8/CD3 = 0.216–0.288). For those exposed to SACI, no correlation was found between the Ab production efficiency and the CD8/CD3 ratio (r = 0.881, p = 0.12) or the proportion of CD8+ cells (r = 0.808, p = 0.19) in the original sample. These results suggest that the repertoire of B cells responsive to LPS is different at least in part from the repertoire responsive to SACI and that the ratio CD8/CD3 could serve as a practical predictor for Ab production by human lymphocytes stimulated with LPS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cairns E, Germain J St and Bell DA (1985) The in vitro production of anti-DNA antibody by cultured peripheral blood or tonsillar lymphoid cells from normal donors and SLE patients. J Immunol 135: 3839-3844.

    PubMed  CAS  Google Scholar 

  • Croce CM, Linnenbach A, Hall W, Steplewski Z and Koprowski H (1980) Production of human hybridomas secreting antibodies to measles virus. Nature 288: 488-489.

    Article  PubMed  CAS  Google Scholar 

  • Farzad Z, Cochran AJ, McBride WH, Gray JD, Wong V and Morton DL (1990) Lymphocyte subset alterations in nodes regional to human melanoma. Cancer Res 50: 3585-3588.

    PubMed  CAS  Google Scholar 

  • Forsgren A, Svedjelund A and Wigzell H (1976) Lymphocyte stimulation by protein A of Staphylococcus aureus. Eur J Immunol 6: 207-213.

    PubMed  CAS  Google Scholar 

  • Foung S, Perkins S, Kafadar K, Gessner P, and Zimmermann U (1990) Development of microfusion techniques to generale human hybridomas. J Immunol Methods 134: 35-42.

    Article  PubMed  CAS  Google Scholar 

  • Haspel MV, McCabe RP, Pomato N, Janesch NJ, Knowlton JV, Peters LC, Hoover Jr HC and Hanna Jr MG (1985) Generation of tumor cell-reactive human monoclonal antibodies using peripheral blood lymphocytes from actively immunized colorectal carcinoma patients. Cancer Res 45: 3951-3961.

    PubMed  CAS  Google Scholar 

  • Ho M-K, Rand N, Murray J, Kato K and Rabin H (1985) In vitro immunization of human lymphocytes. J Immunol 135: 3831-3838.

    PubMed  CAS  Google Scholar 

  • Imam A, Drushella MM, Taylor CR and Tökés ZA (1985) Generation and immunohistological characterization of human monoclonal antibodies to mammary carcinoma cells. Cancer Res 45: 263-271.

    PubMed  CAS  Google Scholar 

  • Jahn S, Grunow R, Kiessig ST, Specht U, Matthes H, Hiepe F, Hlinak A and von Baehr R (1988) Establishment of human Ig producing heterohybridomas by fusion of mouse myeloma cells with human lymphocytes derived from peripheral blood, bone marrow, spleen, lymph node, and synovial fluid. J Immunol Methods 107: 59-66.

    Article  PubMed  CAS  Google Scholar 

  • Kan-Mitchell J, Imam A, Kempf RA, Taylor CR and Mitchell MS (1986) Human monoclonal antibodies directed against melanoma tumor-associated antigens. Cancer Res 46: 2490-2496.

    PubMed  CAS  Google Scholar 

  • Kipps TJ and Duffy SF (1991) Relationship of the CD5 B cell to human tonsillar lymphocytes that express autoantibody-associated cross-reactive idiotypes. J Clin Invest 87: 2087-2096.

    PubMed  CAS  Google Scholar 

  • Kjeldsen T, Rasmussen BB, Rose C and Zeuthen J (1988) Humanhuman hybridomas and human monoclonal antibodies obtained by fusion of lymph node lymphocytes from breast cancer patients. Cancer Res 48: 3208-3214.

    PubMed  CAS  Google Scholar 

  • Nakashima M, Watanabe T, Koprowski H and Steplewski Z (1996) HLA-B-restricted, CD8+ cytolytic human T cell clones derived from a melanoma-invaded lymph node. Hybridoma 15: 147-154.

    Article  PubMed  CAS  Google Scholar 

  • Nozawa S, Narisawa S, Kojima K, Sakayori M, Iizuka R, Mochizuki H, Yamauchi T, Iwamori M and Nagai Y (1989) Human monoclonal antibody (HMST-1) against lacto-series type 1 chain and expression of the chain in uterine endometrial cancers. Cancer Res. 49: 6401-6406.

    PubMed  CAS  Google Scholar 

  • Ringdén O and Rynnel-Dagöö B (1978) Activation of human B and T lymphocytes by protein A of Staphylococcus aureus. Eur J Immunol 8: 47-52.

    PubMed  Google Scholar 

  • Ruuskanen O, Pittard III WB, Miller K, Pierce G, Sorensen RU and Polmar SH (1980) Staphylococcus aureus Cowan I-induced immunoglobulin production in human cord blood lymphocytes. J Immunol 125: 411-413.

    PubMed  CAS  Google Scholar 

  • Schuurman RKB, Gelfand EW and Dosch H-M (1980) Polyclonal activation of human lymphocytes in vitro. J Immunol 125: 820-826.

    PubMed  CAS  Google Scholar 

  • Siroka K (1987) Human monoclonal antibodies to cancer cells. In: Strelkauskas AJ (ed.) Human hybridomas. Marcel Dekker Inc., New York, pp. 159-181.

    Google Scholar 

  • Tsujitani S, Nakashima M, Watanabe T, Kaibara N, Koprowski H and Steplewski Z (1995) Cytokine combinations for induction of antigen-specific cytolytic T lymphocytes from peripheral blood lymphocytes. Anticancer Res 15: 655-660.

    PubMed  CAS  Google Scholar 

  • Umetsu DT and Geha RS (1987) In vitro production of antibody in culture of human peripheral blood lymphocytes. Methods in Enzymol 150: 309-316.

    Article  CAS  Google Scholar 

  • Warenius HM, Taylor JW, Durack BE and Cross PA (1983) The production of human hybridomas from patients with malignant melanoma. The effect of pre-stimulation of lymphocytes with pokeweed mitogen. Eur J Cancer Clin Oncol 19: 347-355.

    Article  PubMed  CAS  Google Scholar 

  • Wright SD, Ramos RA, Tobias PS, Ulevitch RJ and Mathison JC (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433.

    PubMed  CAS  Google Scholar 

  • Yoshinari K, Arai K, Kimura H, Matsumoto K and Yamaguchi Y (1995) Long-term production of human monoclonal antibodies by heterohybridomas. J Immunol Methods 186: 17-25.

    Article  PubMed  CAS  Google Scholar 

  • Yoshinari K, Arai K, Kimura H, Matsumoto K and Yamaguchi Y (1996) Efficient production of IgG human monoclonal antibodies by lymphocytes stimulated by lipopolysaccharide, pokeweed mitogen, and interleukin 4. In Vitro Cell Dev Biol Animal 32: 372-377.

    CAS  Google Scholar 

  • Ziegler-Heitbrock HWL and Ulevitch RJ (1993) CD14: cell surface receptor and differentiation marker. Immunol Today 121: 121-125.

    Article  Google Scholar 

  • Zimmermann U, Gessner P, Schnettler R, Perkins S and Foung SKH (1990) Efficient hybridization of mouse-human cell lines by means of hypo-osmolar electrofusion. J Imunol Methods 134: 43-50.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Diagnostics R&D Department, Asahi Chemical Industry Co. Ltd., Ohito-cho, Shizuoka, 410-2321, Japan

    Kaoru Yoshinari & Kenji Arai

  2. Department of Applied Chemistry, Kanagawa Institute of Technology, Shimoogino, Atsugi-shi, Kanagawa, 243-0292, Japan

    Kunio Matsumoto

  3. Division of Chest Diseases, Chiba Cancer Center, Nitona-cho, Chuo-ku, Chiba, 260-0801, Japan

    Hideki Kimura

Authors
  1. Kaoru Yoshinari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kenji Arai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kunio Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hideki Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kaoru Yoshinari.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yoshinari, K., Arai, K., Matsumoto, K. et al. Relationship between antibody productivity by activated human lymph node lymphocytes from lung cancer patients and lymphocyte subsets. Cytotechnology 29, 229–236 (1999). https://doi.org/10.1023/A:1008052326963

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008052326963

Search

Navigation