Abstract
Reduced growth of plants and excessive generalised and spotty yellowing of leaves occurred in ornamental crotons that were infected with a geminivirus, which was detected by spot hybridization tests using a cloned probe derived from DNA-A of acalypha yellow mosaic geminivirus. The croton virus was transmitted by the whitefly,Bemisia tabaci, toNicotiana tabacum andAcalypha indica, in which it caused leaf curl and yellow mosaic, respectively, but it was not transmitted to five other species. Infected crotons may therefore serve as reservoirs of a geminivirus that is able to infect a crop species.
References
Chenulu VV and Phatak HC (1965) Yellow mosaic ofAcalypha indica L. — A new whitefly transmitted viras disease from India. Current Science 34: 321–322
Dellaporta SJ, Wood J and Hicks JB (1983) A plant DNA mini preparation: Version II. Plant Molecular Biology Reporter 1: 19–21
Feinberg AP and Vogelstein B (1983) A technique for radio labelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132: 6–12
Harrison BD (1985) Advances in geminivirus research. Annual Review of Phytopathology 23: 55–82
Pal BP and Tandon RK (1937) Types of tobacco leaf curl in northern India. Indian Journal of Agricultural Science 7: 363–393
Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn. New York: Cold Spring Harbor Laboratory
Srivastava KM, Dwadash Shreni VC, Srivastava BN and Singh BP (1977) Zinnia yellow net disease — transmission, host range and agent — vector relationship. Plant Disease Reporter 61: 550–554
Srivastava KM, Raizada RK, Srivastava A, Chandra G and Singh BP (1992) Rapid generation of probe for a ssDNA plant viras directly from total DNA of infected tissue. Current Science 62: 481–482
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Raj, S.K., Srivastava, K.M. & Singh, B.P. Evidence from nucleic acid hybridization tests for geminivirus infection of ornamental crotons in India. Eur J Plant Pathol 102, 201–203 (1996). https://doi.org/10.1007/BF01877107
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01877107