Abstract
Four somaclonal variants regenerated from adventitious buds of the apple variety Greensleeves were preselected on the basis of their reduced fire blight susceptibility. The present study aimed at assessing precisely their level of fire blight resistance through various inoculation techniques (on in vitro leaves and microcuttings, on greenhouse plants and in field conditions). Overall results of these tests indicated that one clone (R 46/3) was clearly less susceptible than the control. This clone was also characterized as a ‘spur’ variant, with a reduced growth which can explain its limited susceptibility to fire blight. A second clone (R 20/63) was slightly less susceptible than the control in greenhouse and field tests, but this low level of resistance was overcome by high concentrations of inoculum. The absence of variation in chromosome number and isozyme patterns confirmed the genetic trueness-to-type of these four somaclones.
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Chevreau, E., Brisset, M., Paulin, J. et al. Fire blight resistance and genetic trueness-to-type of four somaclonal variants from the apple cultivar Greensleeves. Euphytica 104, 199–205 (1998). https://doi.org/10.1023/A:1018673813980
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DOI: https://doi.org/10.1023/A:1018673813980