Summary
Host plant genetics of N2 fixation in the cowpea (Vigna unguiculata (L.) Walp.) — Rhizobium symbiosis was investigated in the field with a mixed strain inoculum and in the greenhouse with a single strain inoculum. Five cowpea genotypes, including ‘H-Brown Crowder’ and ‘L-Bush Purple Hull’, were used to generate populations for genetic studies. Diallel analysis for general combining ability (GCA), specific combining ability (SCA) and reciprocal effects was carried out for the N2 fixation variables nitrogenase activity, nodule number, nodule weight, and top dry weight, in the field study. Generation mean analysis and heritability estimates were performed on the greenhouse population which included P1 (‘H-Brown Crowder’), P2 (‘L-Bush Purple Hull’), F1, F1′. F2, BC1, and BC2. Positive and significant correlations were observed between nitrogenase activity and both nodule weight and nodule number. Correlation coefficients between top dry weight and the other 3 variables were not significant. Estimates of SCA were highly significant for all variables except top dry weight, while those of GCA were significant only for nodule weight. Generation mean analysis revealed that additive gene action was more prominent than dominance and interallelic gene action for nodule number and nitrogenase activity, while the opposite was true for nodule weight and top dry weight. Narrow sense heritability estimates were moderately high for nodule number (0.55) and nitrogenase activity (0.62), and low for nodule weight (0.39) and top dry weight (0.17).
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References
Caldwell, B. E., 1966. Inheritance of a strain-specific ineffective nodulation in soybean. Crop Sci. 6: 427–428.
El-Sherbeeny, M. H., D. A., Lawes & L. R., Mytton, 1977. Symbiotic variability in Vicia faba. 2. Genetic variation in Vicia faba. Euphytica 26: 377–383.
Food and Agriculture Organization, United Nations, 1981. Production Yearbook, 1980.
Gamble, E. E., 1962. Gene effects in corn. I. Separation and relative importance of gene effects for yield. Can. J. Plant Sci. 42: 339–398.
Gibson, A. H., 1969. Genetic control of strain-specific ineffective nodulation in Trifolium subterraneum L. Aust. J. Agric. Res. 15: 37–49.
Gorbet, D. W. & J. C., Burton, 1979. A non-nodulating peanut. Crop Sci. 19: 727–728.
Graham, P. H. & J. C., Rosas, 1977. Growth and development of indeterminate bush and climbing cultivars of Phaseolus vulgaris L. inoculated with Rhizobium. J. Agri. Sci. Camb. 88: 503–508.
Graham, P. H., 1979. Influence of temperature on growth and nitrogen fixation in cultivars of Phaseolus vulgaris L., inoculated with Rhizobium. J. Agri. Sci. Camb. 93: 365–370.
Griffing, B., 1956. Concept of general and specific combining ability in relation to diallel crossing systems. Aust. J. Biol. Sci. 9: 463–493.
Holl, F. B., 1975. Host plant control of the inheritance of dinitrogen fixation in the Pisum-Rhizobium symbiosis. Euphytica 24: 767–770.
Holl, F. B. & T. A., Larue, 1975. Genetics of legume host plants. In: W. E., Newton & C. J., Nyman (Eds), Nitrogen fixation. Vol. 2. Washington State Univ. Press, Pullman, Washington, p. 391–399.
Mather, K. & J. L., Jinks, 1977. Introduction to biometrical genetics. Cornell Univ. Press, Ithaca, New York.
Nutman, P. S., 1949. Nuclear and cytoplasmic inheritance of resistance to infection by nodule bacteria in red clover. Heredity 3: 263–291.
Nutman, P. S., 1954. Symbiotic effectiveness in nodulated red clover. II. A major gene for ineffectiveness in the host. Heredity 8: 47–60.
Nutman, P. S., 1957. Symbiotic effectiveness in nodulated red clover, III. Further studies on inheritance of ineffectiveness in the host. Heredity 11: 157–173.
Nutman, P. S., 1968. Genetic effectiveness in nodulated red clover. V. The n and d factors for ineffectiveness. Heredity 23: 537–551.
Paterson, D. R. & H. T. Blackhurst, 1958. Some effects of irrigation, fertilizer, and variety on the yield of southernpeas. Tex. Agri. Exp. Sta. Prog. Rept. 2021.
Peterson, M. A. & D. K., Barnes, 1981. Inheriuance of ineffective nodulation and non-nodulation traits in alfalfa. Crop Sci. 21: 611–616.
Rowe, K. E. & W. L., Alexander, 1980. Computations for estimating the genetic parameters in joint-scaling tests. Crop Sci. 20: 109–110.
Vest, G., 1970. Rj3, a gene conditioning ineffective nodulation in soybean. Crop Sci. 10: 34–35.
Vest, B. & B. E., Caldwell, 1972. Rj4, a gene conditioning ineffective nodulation in soybean. Crop Sci. 12: 692–693.
Wacek, T. J. & W. J., Brill, 1976. Simple rapid assay for screening nitrogen fixation ability in soybean. Crop Sci. 16: 519–523.
Westermann, D. T. & J. J., Kolar, 1978. Symbiotic N2(C2H2) fixation by bean. Crop. Sci. 18: 986–990.
Williams, L. J. & D. L., Lynch, 1953. Inheritance of a non-nodulating character in soybean. Agron. J. 46: 28–29.
Zary, K. W. & J. C., Miller, Jr., 1980. The influence of genotype on diurnal and seasonal patterns of nitrogen fixation in southernpea [Vigna unguiculata (L.) Walp.]. J. Amer. Soc. Hort. Sci. 105: 699–701.
Zary, K. W., J. C., Miller, Jr., R. W., Weaver, & L. W., Barnes, 1978. Intraspecific variability for nitrogen fixation in southernpea [Vigna unguiculata (L.) Walp.]. J. Amer. Soc. Hort. Sci. 103: 806–808.
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Texas Agricultural Experiment Station Technical Article 20385. Research supported in part by a grant under USAID PASA AG/TAB 610-9-76 (USDA-CSRS-701-15-59).
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Miller, J.C., Zary, K.W. & Fernandez, G.C.J. Inheritance of N2 fixation efficiency in cowpea. Euphytica 35, 551–560 (1986). https://doi.org/10.1007/BF00021864
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DOI: https://doi.org/10.1007/BF00021864