Skip to main content
SpringerLink
Log in

Identification of RAPD markers linked to linolenic acid genes in rapessed

  • Published:
Euphytica Aims and scope Submit manuscript

Summary

The inheritance of the low linolenic acid content (derivated from mutant lines) in rapeseed was investigated. Molecular techniques of gene mapping through RAPD markers were applied on a microspore-derived progeny from a high × low linolenic acid F1 hybrid. ‘Bulked segregant analysis’ made it possible to test rapidly number of RAPD primers. Two linkage groups of 6 markers (72.7 cM and 75.6 cM) were determined. Each corresponded to a major QTL which explained 24% and 30.7% of the total phenotypic variation of the linolenic acid content. It was confirmed that two independant mutations were implied in the low linonenic acid content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arondel V., B. Lemieux, I. Hwang, S. Gibson, H.M. Goodman & C.R. Somerville, 1992. Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science 258: 1353–1354.

    Google Scholar 

  • Brunklaus-Jung E. & G. Röbbelen, 1987. Genetical and physiological investigations on mutants for polyenoic fatty acids in rapeseed (Brassica napus L.) Plant Breeding 98: 9–16.

    Google Scholar 

  • Chen J.L. & W.D. Beversdorf, 1990. A comparison of traditional and haploid-derived breeding populations of oilseed rape (Brassica napus L.) for fatty acid composition of the seed oil. Euphytica 51: 59–65.

    Google Scholar 

  • Doyle J.J. & J.L. Doyle, 1990. Isolation of plant DNA from fresh tissues. Focus 12: 13–15.

    Google Scholar 

  • Ferreira M.E., P.H. Williams & T.C. Osborn, 1994. RFLP mapping of Brassica napus using doubled haploid lines. Theor Appl Genet 89: 615–621.

    Google Scholar 

  • Foisset N., R. Delourme, M.O. Lucas & M. Renard, 1993. Segregation analysis of isozyme markers on isolated microspore-derived embryos in Brassica napus L. Plant Breeding 110: 315–322.

    Google Scholar 

  • Foisset N. & R. Delourme, 1995. Segregation distortion in androgenic plants. In: S. Jain Mohan, S.K. Sopory & R.E. Veilleux (Eds.), In Vitro Haploid Production in Higher Plants. Kluwer Academic Publishers. Dordrecht, The Netherlands, in review.

    Google Scholar 

  • Fossati P., 1994. Les huiles de poisson: actualités scientifiques et médicales. OCL 1(3): 178–180.

    Google Scholar 

  • Haley S.D., L. Afanador & J.D. Kelly, 1994. Selection for monogenic pest resistance traits with coupling- and repulsion-phase RAPD markers. Cell Biology and Molecular Genetics. Crop Sci 34: 1061–1066.

    Google Scholar 

  • Harvey B.L. & R.K. Downey, 1963. The inheritance of erucic acid content in rapeseed (Brassica napus L.). Can J Plant Sci 44: 104–111.

    Google Scholar 

  • Harwood J.L. 1994. Environmental effects on plant lipid metabolism. INFORM 5: 835–839.

    Google Scholar 

  • Hu J., J.H. Quiros, P. Arus, D. Struss & G. Robbelen, 1994. Tagging of a gene determining linolenic acid concentration in rapeseed with DNA-based markers. Cruci Newsl 16: 42–43.

    Google Scholar 

  • Jönsson R. & C. Persson, 1983. Breeding for improved fatty acid composition in rapeseed. Proc 6th Int Rapeseed Conf, Paris, France 1: 311–314.

    Google Scholar 

  • Jourdren, C., P. Barret, D. Brunel, R. Debourne & M. Renard, 1996. Specific molecular marker of the genes controlling line acid content in rapeseed. Theor Appl Genet (in press).

  • Lander E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181.

    Google Scholar 

  • Martin G.B., J.G.K. Williams & S.D. Tanksley, 1991. Rapid identification of markers linked to a Pseudomonas resistance gene in Tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88: 2336–2340.

    Google Scholar 

  • Melchinger A.E., 1990. Use of molecular markers in breeding for oligogenic disease resistance. Plant Breeding 104: 1–9.

    Google Scholar 

  • Michelmore R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci 88: 9828–9832.

    Google Scholar 

  • Miquel F.M. & J.A. Browse, 1994. High-oleate oilseeds fail to develop at low temperature. Plant Physiol 106: 421–427.

    Google Scholar 

  • Morice J., 1979. Les nouvelles varietés de colza. In: Symposium April 1978, Bruxelles. Expose 4, 22 p. Commission Commun Europe, Luxembourg.

    Google Scholar 

  • Pleines S. & W. Friedt, 1989. Genetic control of linolenic acid concentration in seed oil of rapeseed (B. napus L.). Theor Appl Genet. 78: 793–797.

    Google Scholar 

  • Polsoni L., L.S. Kott & W.D. Beversdorf, 1988. Large scale microspore culture technique for mutation-selection studies in Brassica napus. Can J Bot 66: 1681–1685.

    Google Scholar 

  • Powell W., W.T.B. Thomas, D.M. Thompson, J.S. Swanston & R. Waugh, 1992. Association between rDNA alleles and quantitative traits in doubled haploid populations of barley. Genetics 130: 187–194.

    Google Scholar 

  • Röbbelen G. & A. Nitsch, 1975. Genetical and physiological investigations on mutants for polyenoic fatty acids in rapeseed (B. napus L.) I. Selection and description of new mutants. Z Pflanzenzüchtg 75: 93–105.

    Google Scholar 

  • SAS Institute Inc., 1988. SAS Users Guide: Statistics. SAS Institute Cary, N.C.

    Google Scholar 

  • Scarth R., P.B.E. Mcvetty, S.R. Rimmer, & B.R. Stefansson, 1991. Hero summer rape. Can J Plant Sci 71: 865–866.

    Google Scholar 

  • Scarth R., P.B.E. Mcvetty & B.R. Stefansson, 1988. Stellar low linolenic-high linoleic acid summer rape. Can J Plant Sci 68: 509–511.

    Google Scholar 

  • Tanhuanpää P.K., J.P. Vilkki & K.J. Vilkki 1995. Association of a RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.). Genome 38: 414–416.

    Google Scholar 

  • Thies W., 1971. Schnelle und Einfache Analysen der Fettsäurezusammensetzung in Einzelnen Rapskotyledonen. I. Gaschromatographische und papier-Chromatographische Methoden. Z Pflanzenzüchtg 65: 181–202.

    Google Scholar 

  • Williams J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphism and amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531–6535.

    Google Scholar 

  • Yadav N.S., A. Wierzbicki, M. Aegerter, C.S. Caster, L. Pérez-Grau, A.J. Kinney, W.D. Hitz, J. Russel BoothJr, B. Schweiger, K.L. Stecca, S.M. Allen, M. Blackwell, R.S. Reiter, T.J. Carlson, S.H. Russel, K.A. Feldmann, J. Pierce & J. Browse, 1993. Cloning of higher plant ω-3 fatty acid desaturases. Plant Physiol 103: 467–476.

    Google Scholar 

  • Young N.D., D. Zamir, M.W. Ganal & S.D. Tanksley, 1988. Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in Tomato. Genetic 20: 579–585.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Station d'Amélioration des Plantes, INRA, BP 29, F-35650, Le Rheu, France

    Christelle Jourdren, Pierre Barret, Raymonde Horvais, Régine Delourme & Michel Renard

Authors
  1. Christelle Jourdren
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pierre Barret
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raymonde Horvais
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Régine Delourme
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Michel Renard
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jourdren, C., Barret, P., Horvais, R. et al. Identification of RAPD markers linked to linolenic acid genes in rapessed. Euphytica 90, 351–357 (1996). https://doi.org/10.1007/BF00027487

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00027487

Key words

Search

Navigation