Abstract
We have recently shown that a plasmid-borneDissociation (Ds) element can excise from extrachromosomal plasmid DNA and integrate into a plant genome in the presence of theActivator (Ac) transposase.Ds andAc-carrying plasmids were used to co-transformNicotiana plumbaginifolia protoplasts. Transgenic plants were regenerated and analyzed. Here we describe further characterization of the system and discuss its efficiency in terms of DNA transformation and transposon tagging.
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Houba-Hérin, N., Domin, M. & Leprince, AS. Some features about transposition of the maize elementDissociation inNicotiana plumbaginifolia . Genetica 93, 41–48 (1994). https://doi.org/10.1007/BF01435238
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DOI: https://doi.org/10.1007/BF01435238