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Dedifferentiation and redifferentiation of the penis of the freshwater planarian Bdellocephala brunnea

  • Regeneration and Asexual Reproduction
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Abstract

Following decapitation of Bdellocephala brunnea Ijima et Kaburaki and subsequent repeated removal of regenerating head-blastemas, the copulatory apparatus degenerated within 40–60 days. The copulatory apparatus disintegrated into cell clusters that further divided into separate neoblast-like cells that, in turn, eventually transformed into ordinary parenchymal cells by 60 days after decapitation. When the head of worms with a degenerate copulatory apparatus was allowed to regenerate by discontinuing excision of the head blastema, a new copulatory apparatus differentiated, ostensibly from the dedifferentiated cells. The neoblast-like cells appear to participate in the redifferentiation of the penis and of other parts of the copulatory apparatus.

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References

  • Baguñà, J., E. Saló & C. Auladell, 1989. Regeneration and pattern formation in planarians III. Evidence that neoblasts are totipotent stem cells and the source of blastema cells. Development 107: 77–86.

    Google Scholar 

  • Brøndsted, H. V., 1969. Planarian regeneration. Pergamon Press, Oxford, pp. 1–276.

    Google Scholar 

  • Chandebois, R., 1976. Histogenesis and morphogenesis in planarian regeneration. Monogr. Dev. Biol. 11, A. Wolsky (ed.), Karger, Basel, New York, 182 pp.

    Google Scholar 

  • Coward, S. J., 1969. Regeneration in planarians: some unresolved questions and problems. J. Biol. Psychol. 11: 15–19.

    Google Scholar 

  • Coward, S. J. & E. D. Hay, 1972. Fine structure of cells involved in physiological and reconstitutive regeneration in planarians. Anat. Rec. 172: 296. [Abstract]

    Google Scholar 

  • Curtis, W. C., 1902. The life history, the normal fission and the reproductive organs of Planaria maculata. Proc. Boston Soc. Nat. Hist. pp515–559.

  • Curtis, W. C. & L. M. Schulze, 1934. Studies upon regeneration. 1. The contrasting powers of regeneration in planaria and procotyla. J. Morphol. 55: 477–512.

    Google Scholar 

  • Flickinger, R. A., 1964. Isotopic evidence for a local origin of blastema cells in regenerating planaria. Exp. Cell Res. 34: 403–406.

    Google Scholar 

  • Ghirardelli, E., 1965. Differentiation of the germ cells and regeneration of the gonads in planarians. In V. Kiortsis & H. A. L. Trampush (eds), Regeneration in animals and related problems. Regeneration in animals, 117. North-Holland Publ. Co., Amsterdam: 177–184.

    Google Scholar 

  • Gremigni, V., 1981. The problem of cell totipotency, dedifferentiation and transdifferentiation in Turbellaria. Hydrobiologia. 84: 171–179.

    Google Scholar 

  • Gremigni, V., 1988. Planarian regeneration: an overview of some cellular mechanisms. Zool. Sci. 5: 1153–1163.

    Google Scholar 

  • Gremigni, V. & C. Miceli, 1980. Cytophotometric evidence for cell ‘transdifferentiation’ in planarian regeneration. Wilhelm Roux's Arch. Dev. Biol. 188: 107–113.

    Google Scholar 

  • Gremigni, V. & I. Puccinelli, 1977. A contribution to the problem of the origin of blastema cells in planarians: a karyological and ultrastructural investigation. J. exp. Zool. 199: 57–72.

    Google Scholar 

  • Hay, E. D., 1966. Regeneration. Holt, Rinhart and Winston, New York.

    Google Scholar 

  • Hay, E. D., 1968. Dedifferentiation and metaplasia in vertebrate and invertebrate regeneration. In H. Ursprung (ed.), The stability of the differentiated state. Springer-Verlag, Berlin: 85–108.

    Google Scholar 

  • Hyman, L. H., 1951. The invertebrates. Vol. II. Platyhelminthes and Rhyncocoela. McGraw-Hill, New York, 551 pp.

    Google Scholar 

  • Lang, A., 1912. Über Regeneration bei Planarien. Arch. Mikrosk. Anat. 79: 361–426.

    Google Scholar 

  • Lender, T., 1962. Factors in morphogenesis of regenerating freshwater planaria. In M. Abercrombie & J. Brachet (eds), Advances in morphogenesis, Vol. II. Academic Press, New York: 305–331.

    Google Scholar 

  • Pedersen, K. J., 1972. Studies on regeneration blastemas of the planarian Dugesia tigrina with special reference to differentiation of the muscle-connective tissue filament system. Wilhelm Roux's Arch. Dev. Biol. 169: 134–169.

    Google Scholar 

  • Puccinelli, I. & V. Gremigni, 1977. Sulla presenza nei blastemi di planarie di cellule provenienti dal territorio gonadico femminile. Accad. Naz. Lincei. 63: 588–592.

    Google Scholar 

  • Rose, C. & S. Shostak, 1968. The transformation of gastrodermal cells to neoblasts in regenerating Phagocata gracilis (Leidy). Exp. Cell Res. 50: 553–561.

    Google Scholar 

  • Satô, T., 1968. A modified method for lead staining of thin sections. J. electron Microsc. 17: 158–159.

    Google Scholar 

  • Steinman, P., 1908. Untersuchungen über dass Verhalten des Verdaungssystems bei der Regeneration der Tricladen. Arch. Entwicklungsmech. Org. (Wilhelm Roux) 25: 523–568.

    Google Scholar 

  • Steinman, P., 1925. Das Verhalten der Zellen und Gewebe im regenerierenden Tricladen-Körper. Verh. Naturf. Ges. Basel 36: 133–162.

    Google Scholar 

  • Teshirogi, W., 1955. On the regeneration of a turbellarian, Bdellocephala brunnea, especially its frequencies of head, tail, pharynx and genital organ formation. Zool. Mag. (Tokyo) 64: 294–299. [In Japanese with English summary]

    Google Scholar 

  • Teshirogi, W., 1962. Dynamic morphological change and time-table during the regeneration of the turbellarian Bdellocephala brunnea. Sci. Rep. Hirosaki Univ. 9: 21–48.

    Google Scholar 

  • Teshirogi, W., 1986. On the origin of neoblasts in freshwater planarians (Turbellaria). Hydrobiologia. 132: 207–216.

    Google Scholar 

  • Teshirogi, W., 1987. Biology of planarians — foundation, application and experiments. Kyoritsu Shuppan Inc., Tokyo: 65–83. [In Japanese]

  • Teshirogi, W. & H. Fujiwara, 1970. Some experiments on regeneration and differentiation of genital organ in a freshwater planarian, Bdellocephala brunnea. Sci. Rep. Hirosaki Univ. 17: 38–49.

    Google Scholar 

  • Teshirogi, W., M. Kudoh & W. Yoshida, 1991. On the totipotency of dedifferentiated cells originated from copulatory apparatus in the planarian. Zool. Sci. (in press).

  • Wolff, E., 1962. Recent researches on the regeneration of planaria. In D. Rudnick (ed.), Regeneration. The Ronald Press, New York: 53–84.

    Google Scholar 

  • Wolff E. & F. Dubois, 1948. Sur la migration des cellules de régénération chez les planaires. Revue suisse Zool. 55: 218–227.

    Google Scholar 

  • Woodruff, L. & A. L. Burnett, 1965. The origin of the blastema cells in Dugesia tigrina. Exp. Cell Res. 38: 295–305.

    Google Scholar 

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Kudoh, M., Teshima, H. & Teshirogi, W. Dedifferentiation and redifferentiation of the penis of the freshwater planarian Bdellocephala brunnea . Hydrobiologia 227, 47–56 (1991). https://doi.org/10.1007/BF00027580

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