Abstract
Planarian head extract was fractionated and the fractions were assayed for their effect on cultured cells and planarian regeneration. One fraction (molecular weight larger than 10 000; unadsorbable by DEAE-Sephadex, CM-Sephadex and Con A-Sepharose; and precipitable by ammonium sulfate) inhibited the growth of both Neuro 2a and PC-12 cell lines as well as planarian head- regeneration. This effect was specific for head-regeneration (regeneration of tails was not influenced), trypsin sensitive, reversible and stable after heat-treatment at 80° C for 30 min.
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Shinozawa, T., Shiozaki, S., Ezaki, M. et al. Regulation factor for planarian regeneration. Hydrobiologia 305, 247–253 (1995). https://doi.org/10.1007/BF00036397
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DOI: https://doi.org/10.1007/BF00036397