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Supercritical fluid CO2 extraction accelerates isolation of humic acid from livePilayella littoralis (Phaeophyta)

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Abstract

Supercritical fluid CO2 extraction of the live, dried algaPilayella littoralis efficiently removes non-humic substances in hours instead of the days required for sequential extraction with ether, acetone, ethanol and dioxane. Completion of extraction (including aqueous removal of alginic acids) with either preliminary in a standard procedure gives humic acid samples with closely comparable properties.

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References

  • Bartle KD, Martin TG, Williams DF (1975) Chemical nature of a supercritical gas extract of coal at 350°C. Fuel 54: 226–235.

    Google Scholar 

  • Baumeister M, Edel E (1980) Ethanol-water pulping. Papier (Darmstadt) 34: V9–V18.

    Google Scholar 

  • Chen Y, Senesi N, Schnitzer M (1977) Information provided on humic substances by E4/E6 ratios. Soil Sci. Soc. Am. J. 41: 352–358.

    Google Scholar 

  • Chen Y, Senesi N, Schnitzer M (1978) Chemical and physical characteristics of humic and fulvic acids extracted from soils of the mediterreanean region. Geoderma 20: 87–104.

    Google Scholar 

  • Firth FE (1969) Seaweeds in commerce. In: Encyclopedia of Marine Resources. Van Nostrand-Reinhold, New York.

    Google Scholar 

  • Flaig W, Beutelspacher H, Rietz E (1975) Chemical composition and physical properties of humic substances. In Gieseking JE (ed.), Soil Components, Volume 1, Organic Components. Springer-Verlag, New York: 4–7.

    Google Scholar 

  • Ghabbour EA, Khairy AH, Cheney DP, Gross V, Davies G, Gilbert TR, Zhang X (1994) Isolation of humic acid from the brown algaPilayella littoralis. J. appl. Phycol. 6: 459–468.

    Google Scholar 

  • Hubert P, Vitzthum OG (1978) Fluid extraction of hops, spices and tobacco with supercritical gases. Angew. Chem. Int. Ed. Engl. 17: 710–715.

    Google Scholar 

  • Pierce Jr RH, Felbeck Jr GT (1972) A comparison of three methods of extracting organic matter from soils and marine sediments. Proc. Int. Meeting Humic Substances, Niewersluis: 217–232.

  • Radwan A (1996) Isolation, characterization and processing of humic acids from live plants. Doctoral dissertation. Northeastern University, Boston, USA.

    Google Scholar 

  • Read RC, Prausnitz JM, Sherwood TK (1977) The Properties of Gases and Liquids. McGraw-Hill, New York, 3rd edn., 633.

  • Scheffer F, Ziechmann W, Pawelke G (1960) Schonende Gewinnung naturlicher Huminstoffen mit Hilfe milder organischer Losungsmittel. Z. Pflanzenemahr Dung. Bodenkd. 90: 58–67.

    Google Scholar 

  • Senesi N, Miano TM (eds), (1994) Humic substances in the Global Environment: Implications for Human Health, Elsevier, New York.

    Google Scholar 

  • Schnitzer M, Preston CM (1987) Supercritical gas extraction of a soil with solvents of increasing polarities. Soil Sci. Soc. Am. J. 51: 639–646.

    Google Scholar 

  • Schulten HR, Schnitzer M (1991) Supercritical carbon dioxide extraction of long chain aliphatics from two soils. Soil Sci. Soc. Am. J. 55: 1603–1611.

    Google Scholar 

  • Spiteller M (1982) A new procedure for the extraction of organic matter from soils by supercritical gases. Z. Pflanzenemahr Dung. Bodenkd. 145: 483–492.

    Google Scholar 

  • Spiteller M (1985) Extraction of soil organic matter by supercritical fluids. Org. geochem. 8: 111–113.

    Google Scholar 

  • Spiteller M, Ashauer A (1982) A new procedure for the extraction of organic matter from soils by supercritical gases. Part 2. Z. Pflanzenemahr Dung. Bodenkd. 145: 567–575.

    Google Scholar 

  • Whyte JNC (1988) Extraction of alginic acid from a brown seaweed. In: Lobban CS, Chapman DJ, Kremer BP (eds), Experimental Phycology: A Laboratory Manual, Cambridge University Press, New York, 168–173.

    Google Scholar 

  • Ziechmann W (1993) Humic substances. BI Wissenschaftsverlag, Mannheim, 220 pp, pp. 52–68.

    Google Scholar 

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Radwan, A., Willey, R.J., Davies, G. et al. Supercritical fluid CO2 extraction accelerates isolation of humic acid from livePilayella littoralis (Phaeophyta). J Appl Phycol 8, 545–551 (1996). https://doi.org/10.1007/BF02186334

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  • DOI: https://doi.org/10.1007/BF02186334

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