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Conformational and antigenic properties of SRYD-containing peptide analogues of Leishmania gp63 adhesion site

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Summary

The antigenicity and conformational properties of the Ser-Arg-Tyr-Asp (SRYD) segment (252–255) of the major surface glycoprotein ofLeishmania, gp63, which plays a key role in the parasite-macrophage attachement, are presented. It was found that the antibody recognition, using anti-IASRYDQL antibodies, of the SRYD-containing analogues, Ac-SRYD-NH2 (1), ANIASRYD-NH2 (2), Ac-SRYD (3), SRYD (4) and ANIASRYD (5), is rather similar. The structure of the SRYD moiety in analogues 1 and 2 is characterized by the presence of a type I β-turn, stabilized by the formation of a hydrogen bonding between the C-terminaltrans-carboxamide proton and the Arg-CO and an ionic bridge between arginine and aspartic acid side chains, while the conformation of compounds 3, 4 and 5 is stabilized by an ionic bridge between the arginine side chain and the C-terminal carboxylate group. A common structural motif involving the arginine side chain in an ionic interaction is identified in all the SRYD analogues, which may explain the observed similarities in the antibody recognition of the reported peptides.

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Authors and Affiliations

  1. Department of Chemistry, University of Ioannina, P.O. Box 1186, 45110, Ioannina, Greece

    Maria Sakarellos-Daitsiotis, Eugenia Panou-Pomonis, Constantinos Sakarellos & Vassilios Tsikaris

  2. LCPM, CNRS-URA 494, ENSIC-INPL, BP 451, F-54001, Nancy Cédex, France

    Manh Thong Cung & Michel Marraud

  3. Department of Biochemistry, Hellenic Pasteur Institute, 127 Vasilissis Sofias Avenue, 11521, Athens, Greece

    Athina K. Tzinia & Ketty Soteriadou

Authors
  1. Maria Sakarellos-Daitsiotis
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  2. Eugenia Panou-Pomonis
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  3. Constantinos Sakarellos
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  4. Manh Thong Cung
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  5. Michel Marraud
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  6. Athina K. Tzinia
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  7. Ketty Soteriadou
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  8. Vassilios Tsikaris
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Sakarellos-Daitsiotis, M., Panou-Pomonis, E., Sakarellos, C. et al. Conformational and antigenic properties of SRYD-containing peptide analogues of Leishmania gp63 adhesion site. Lett Pept Sci 3, 200–208 (1996). https://doi.org/10.1007/BF00128108

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  • DOI: https://doi.org/10.1007/BF00128108

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