Summary
Basic cell culture conditions required for execution of cell transformation studies with C3H/10T1/2 mouse embryo fibroblasts are described. These protocols permit assessment of the ability of chemicals to convert nontumorigenic C3H/10T1/2 cells to a tumorigenic phenotype characterized by altered controls upon in vitro cell division. Recent assay modifications that enhance the sensitivity of this focus formation assay and permit the study of multistage transformation processes are noted.
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Boreiko, C.J. Methodology for cell transformation assays with C3H/10T1/2 mouse embryo fibroblasts. Journal of Tissue Culture Methods 10, 165–172 (1986). https://doi.org/10.1007/BF01405079
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DOI: https://doi.org/10.1007/BF01405079