Abstract
Apical segments of embryonic axes of sunflower (Helianthus annuus L.) embryos were submitted to co-culture experiments with a disarmed strain of Agrobacterium tumefaciens, harbouring a plasmid coding for the marker enzyme β-glucuronidase. The expression patterns of this marker were analysed at different developmental stages of the regenerated shoots. The results are consistent with the hypothesis that transformed shoots originate from transformation events that have occurred within the existing meristems. Two of the resulting chimaeric plants have been analysed in detail, and some representative gene integration patterns are presented.
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Bidney D, Scelonge C, Martich J, Burrus M, Sims L, Huffman G: Microprojectile bombardment of plant tissue increases transformation frequency by Agrobacterium tumefaciens. Plant Mol Biol 18: 301–313 (1992).
Christou P, McCabe DE: Prediction of germ-line transformation events in chimeric Ro transgenic soybean plantlets using tissue-specific expression patterns. Plant J 2: 283–290 (1992).
Dellaporta SL, Wood L, Hicks JB: A plant DNA minipreparation: Version II. Plant Mol Biol Rep 1: 19–21 (1983).
Fischer C, Klethi P, Hahne G: Protoplasts from cotyledon and hypocotyl of sunflower (Helianthus annuus L.): shoot regeneration and seed production. Plant Cell Rep 11: 632–636 (1992).
Jegla DE, Sussex IM: Cell lineage patterns in the shoot meristem of the sunflower embryo in the dry seed. Devel Biol 131: 215–225 (1988).
Jefferson RA: Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387–405 (1987).
Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucosidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
Knittel N, Gruber V, Hahne G, Lénée P: Transformation of sunflower (Helianthus annuus L.): a reliable protocol. Plant Cell Rep 14: 81–86 (1994).
Malone-Schonenberg J, Scelonge CJ, Burrus M, Bidney DL: Stable transformation of sunflower using Agrobacterium and split embryonic axis explants. Plant Sci 103: 199–207 (1994).
McCabe DE, Swain WF, Martinell BJ, Christou P. Stable transformation of soybean (Glycine max) by particle acceleration. Bio/technology 6: 923–925 (1988).
Meyer P: Understanding and controlling transgene expression. Trends Biotechnol 13: 332–337 (1995).
Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497 (1962).
Sambrook J, Fritsch EF, Maniatis T: Molecular cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Schrammeijer B, Sijmons PC, van den Elzen PJM, Hoekema A: Meristem transformation of sunflower via Agrobacterium. Plant Cell Rep 9: 55–60 (1990).
Smith RH, Gould JH, Ulian EC: Method for transforming plants via the shoot apex. In: World Intellectual Property Organization. Plant Physiol, Suppl (1988).
Potrykus I: Gene transfer to plants: assessment of published approaches and results. Annu Rev Plant Physiol Plant Mol Biol 42: 205–225 (1991).
Töpfer R, Schell J, Steinbiß H-H: Versatile cloning vectors for transient gene expression and direct gene transfer in plant cells. Nucl Acids Res 16: 8725 (1988).
Ulian EC, Smith RH, Gould JH, McKnight TD: Transformation of plants via the shoot apex. In Vitro Cell Devel Biol 24: 951–954 (1988).
Vancanneyt G, Schmidt R, O'Connor-Sanchez A, Willmitzer L, Rocha-Soca M: Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet 220: 245–250 (1990).
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Burrus, M., Molinier, J., Himber, C. et al. Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: transformation patterns. Mol Breeding 2, 329–338 (1996). https://doi.org/10.1007/BF00437911
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DOI: https://doi.org/10.1007/BF00437911