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Cytoplasmic poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase in AEV-transformed chicken erythroblasts

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Abstract

Poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase activities were both investigated in chicken erythroblasts transformed by Avian Erythroblastosis Virus. Respectively 21% and 58% of these activities were found to be present in the post-mitochondrial supernatant (PMS). Fractionation of the PMS on sucrose gradients and poly(A+) mRNA detection by hybridization to [3H] poly(U) show that cytoplasmic poly(ADP-ribose) polymerase is exclusively localized in free mRNP. The glycohydrolase activity sedimented mostly in the 6 S region but 1/3 of the activity was in the free mRNP zone. Seven poly(ADP-ribose) protein acceptors were identified in the PMS in the Mr 21000–120000 range. The Mr 120000 protein corresponds to automodified poly(ADP-ribose) polymerase. A Mr 21000 protein acceptor is abundant in PMS and a Mr 34000 is exclusively associated with ribosomes and ribosomal subunits. The existence of both poly(ADP-ribose) polymerase and glycohydrolase activities in free mRNP argues in favour of a role of poly(ADP-ribosylation) in mRNP metabolism. A possible involvement of this post translational modification in the mechanisms of repression-derepression of mRNA is discussed.

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Abbreviations

ADP-ribose:

adenosine (5′) diphospho(5)-β-D ribose

poly(ADP-ribose):

polymer of ADP-ribose

mRNP:

messenger ribonucleoprotein particles

PMSF:

phenylmethylsulfonyl fluoride

LDS:

lithium dodecyl sulfate

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Centre de Neurochimie du CNRS 5, rue Blaise Pascal, 67084, Strasbourg Cedex, France

    H. Thomassin, C. Maniez & P. Mandel

  2. Service de Biochimie de la Différenciation, Institut Jacques Monod, CNRS, Université Paris VII, Tour 43, 2 place Jussieu, 75251, Paris Cedex 05, France

    C. Martins de Sa & K. Scherrer

Authors
  1. H. Thomassin
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  2. C. Martins de Sa
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  3. K. Scherrer
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  4. C. Maniez
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  5. P. Mandel
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Thomassin, H., de Sa, C.M., Scherrer, K. et al. Cytoplasmic poly(ADP-ribose) polymerase and poly(ADP-ribose) glycohydrolase in AEV-transformed chicken erythroblasts. Mol Biol Rep 13, 35–44 (1988). https://doi.org/10.1007/BF00805637

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  • DOI: https://doi.org/10.1007/BF00805637

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