Skip to main content
SpringerLink
Log in

Substrate depletion during formation of aflatoxin and kojic acid on corn inoculated with Aspergillus flavus

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Depletion of sugar and starch carbon sources and concomitant formation of secondary fungal metabolites, aflatoxin and kojic acid, were examined in growing corn inoculated with Aspergillus flavus. Kernels from control and inoculated ears were removed and analyzed after 16, 24, 48, 96 and 168 hrs.

Reducing sugars were not significantly different for inoculated and control non-inoculated samples, but after 168 hrs (seven days) starch content was 20% lower in inoculated than in control samples. Kojic acid was detected before aflatoxins formed. Kojic acid, the oxidized product of kojic acid, and aflatoxin were all present in samples two days from inoculation. The formation of this oxidation product may influence toxin levels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cuculu AF, Lee LS, Pons WA Jr: Relationship of physical appearance of individual cottonseed to aflatoxin content. J Am Oil Chem Soc 54:235A-237A, 1977.

    Google Scholar 

  2. Dickens JW, Whitaker TB: Sampling, BGYF, and aflatoxin analysis in corn. Southern Cooperative Series Bulletin 279:35–37, 1983.

    Google Scholar 

  3. Fleming SE, Reichert RD: Note on a modified method for the quantitative determination of starch. Cereal Chem 57:153–154, 1980.

    Google Scholar 

  4. Lee LS, Conkerton EJ, Ehrlich KC, Ciegler A: Reducing sugars and minerals from lint of unopened cotton bolls as a substrate for aflatoxin and kojic acid synthesis by Aspergillus flavus. Phytopathology 73:734–736, 1983.

    Google Scholar 

  5. Lee LS, Lillehoj EB, Kwolek WF: Aflatoxin distribution in individual corn kernels from intact ears. Cereal Chem 57:340–342, 1980.

    Google Scholar 

  6. Marsh PB, Simpson ME, Ferretti RJ, Merola GV, Donoso J, Craig GO, Trucksess MW, Work PS: Mechanism of formation of a fluorescence in cotton fiber associated with aflatoxins in seeds at harvest. J Agric Food Chem 17:468–472, 1969.

    Google Scholar 

  7. Malik VS: Genetics and biochemistry of secondary fungal metabolites. Advances in Appl Microbiol 28:28–113, 1982.

    Google Scholar 

  8. Miller GL, Slater R, Biozgalis R, Blum R: Application of different colorimetric tests to cellodextrins. Anal Biochem 2:521–528, 1961.

    Google Scholar 

  9. Shotwell OL, Goulden ML, Hesseltine CW: Aflatoxin: Distribution in contaminated corn. Cereal Chem 51:492–496, 1974.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA, USA

    L. S. Lee, F. W. Parrish & T. J. Jacks

Authors
  1. L. S. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. W. Parrish
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. J. Jacks
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, L.S., Parrish, F.W. & Jacks, T.J. Substrate depletion during formation of aflatoxin and kojic acid on corn inoculated with Aspergillus flavus . Mycopathologia 93, 105–107 (1986). https://doi.org/10.1007/BF00437741

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00437741

Keywords

Search

Navigation