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Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment

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Abstract

Gold particles coated with β-glucuronidase (GUS) mRNA with a 5′ cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum), freesia (Freesia refracta) and tulip (Tulipa gesneriana). A fluorometric assay for the GUS activity indicated that in vitro synthesized GUS mRNA introduced into these pollen cells by particle bombardment was successfully expressed. GUS activity in extracts of the bombarded lily pollen became detectable fluorometrically within 30 min after bombardment, peaked at 6 h, then gradually decreased. This activity changed as a function of the developmental stage of the pollen cell of lily.

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Author notes

  1. Atsushi Sakamoto

    Present address: National Institute for Basic Biology, Myodaiji, 444, Okazaki, Japan

Authors and Affiliations

  1. Graduate Department of Gene Science, Faculty of Science, Hiroshima University, 739, Higashi-Hiroshima, Japan

    Toshinori Tanaka, Masahiro Nishihara, Motoaki Seki, Kohei Irifune & Hiromichi Morikawa

  2. Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, 606, Kyoto, Japan

    Atsushi Sakamoto & Kunisuke Tanaka

Authors
  1. Toshinori Tanaka
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  2. Masahiro Nishihara
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  3. Motoaki Seki
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  4. Atsushi Sakamoto
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  5. Kunisuke Tanaka
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  6. Kohei Irifune
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  7. Hiromichi Morikawa
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Tanaka, T., Nishihara, M., Seki, M. et al. Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment. Plant Mol Biol 28, 337–341 (1995). https://doi.org/10.1007/BF00020252

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  • DOI: https://doi.org/10.1007/BF00020252

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