Skip to main content
Log in

Isolation and characterization of a rice cDNA which encodes a ubiquitin protein and a 52 amino acid extension protein

  • Update section
  • Short communication
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

We isolated a rice cDNA clone encoding the ubiquitin protein fused to a ribosomal protein. This clone encodes a single ubiquitin polypeptide and extension protein of 53 amino acids. This extension protein shows a high degree of homology with those of the yeast ubil or ubi2 gene, both of which encode the same protein. Northern blot analysis suggested that the expression pattern of this gene is more similar to other ribosomal protein genes not linked to ubiquitin protein than to the polyubiquitin gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Callis J, Raasch JA, Vierstra RD: Ubiquitin extension proteins of Arabidopsis thaliana—Structure, localization, and expression of theirs promoters in transgenic tobacco. J Biol Chem 265: 12486–12493 (1990).

    Google Scholar 

  2. Callis LP, Shanklin J, Wettern M, Vierstra RD: Sequence of a cDNA from Chlamydomonas reinhardtii encoding a ubiquitin 52 amino acid extension protein. Nucl Acids Res 17: 8377 (1989).

    Google Scholar 

  3. Gausing K, Jensen CB: 2 Ubiquitin long-tail fusion genes arranged as closely spaced direct repeats in barley. Gene 94: 165–171 (1990).

    Google Scholar 

  4. Finley D, Bartel B, Varshavsky A: The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosomal biogenesis. Nature 338: 394–401 (1989).

    Google Scholar 

  5. Genschik P, Parmentier Y, Criqui MC, Fleck J: Sequence of a ubiquitin carboxyl extension protein of Nicotiana tabacum. Nucl Acids Res 18: 4007 (1990).

    Google Scholar 

  6. Hoffman NE, Ko K, Milkowski D, Pichersky E: Isolation and characterization of tomato cDNA and genomic clones encoding the ubiquitin gene ubi3. Plant Mol Biol 17: 1189–1201 (1991).

    Google Scholar 

  7. Ozakaynak E, Finley D, Solomon MJ, Varshavsky A: The yeast ubiquitin genes: a family of natural gene fusions. EMBO J 6: 1429–1439 (1987).

    Google Scholar 

  8. Redman KL, Rechsteiner M: Identification of the long ubiquitin extension as ribosomal protein S27a. Nature 338: 438–440 (1989).

    Google Scholar 

  9. Salveson G, Lloyd C, Farley D: cDNA encoding a human homolog of yeast ubiquitin 1. Nucl Acids Res 15: 5485–5486 (1987).

    Google Scholar 

  10. Uchimiya H, Kido S, Shimazaki T, Aotsuka S, Takamatsu S, Nishi R, Hashimoto H, Matsubayashi Y, Kido N, Umeda M, Kato A: Random sequencing of cDNA libraries reveals a variety of expressed genes in cultured cells of rice (Oryza sativa L.) Plant J 2: 1005–1009 (1992).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nishi, R., Hashimoto, H., Kidou, Si. et al. Isolation and characterization of a rice cDNA which encodes a ubiquitin protein and a 52 amino acid extension protein. Plant Mol Biol 22, 159–161 (1993). https://doi.org/10.1007/BF00039004

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00039004

Key words

Navigation