Abstract
Although the sensitivity of the plasma membrane H+-ATPase to vanadate is well known, the metabolic response of plant cells to vanadate is less well characterised in vivo and its use as an inhibitor in whole plant experiments has had mixed success. Experiments with maize (Zea mays, L.) roots and with purified plasma membrane fractions from the same tissues showed that exposure to vanadate caused: (i) a reduction in the capacity for phosphate uptake; (ii) a reduction in the extractable ATPase activity from the tissue; and (iii) a significant increase in the ATP level. The measurements on the extractable ATPase activity and the ATP level showed that the effect of vanadate developed slowly, apparently reflecting the slow accumulation of intracellular vanadate. The marked effect of vanadate on the ATP level-exposure to 500 μM vanadate for 5 h doubled the ATP content of the roots tips-indicates that there is no stringent control over the ATP level in the roots and that the plasma membrane H+-ATPase activity is likely to have a significant role in determining the ATP level under normal conditions.
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References
BeaugeL 1988 Inhibition of translocation reactions by vanadate. Methods Enzymol. 156, 251–267.
BowmanB J and SlaymanC W 1979 The effects of vanadate on the plasma membrane ATPase ofNeurospora crassa. J. Biol. Chem. 254, 2928–2934.
BrummellD A, HallJ L and ArmstrongC F 1986 Effect of vanadate on microsomal ATPase activity, acidification and auxin stimulated growth in pea and cucumber. Ann. Bot. 57, 727–735.
CantleyL C, CantleyL G and JosephsonL 1978 A characterisation of vanadate interactions with the Na+ K+ ATPase. Mechanistic and regulatory implications. J. Biol. Chem. 253, 7361–7368.
CocucciM, Ballarin-DenteA and MarréM T 1980 Effects of ortho-vanadate on H+ secretion, K+ uptake, electric potential difference and membrane ATPase activities of higher plant tissues. Plant Sci. Lett. 17, 391–400.
CowanD S C, ClarksonD T and HallJ L 1993 A comparison between the ATPase and proton pumping activities of plasma membranes isolated from the stele and cortex ofZea mays roots. J. Exp. Bot. 44, 983–989.
GallagherS R and LeonardR T 1982 Effect of vanadate, molybdate and azide on membrane-associated ATPase and soluble phosphatase activities of corn root. Plant Physiol. 70, 1335–1340.
GranthamJ J and GlynnI M 1979 Renal Na+, K+ ATPase: determinants of inhibition by vanadium. Am. J. Physiol. 236, 530–535.
HooksM A, ClarkR A, NiemanR H and RobertsJ K M 1989 Compartmentation of nucleotides in corn root tips studied by31P NMR and HPLC. Plant Physiol. 89, 963–969.
KimeM J, LoughmanB C, RatcliffeR G and WilliamsR J P 1982 The application of31P nuclear magnetic resonance to higher plant tissue. I. Detection of spectra. J. Exp. Bot. 33, 656–669.
KustinK and ToppenD L 1973 Kinetics of complexation of vanadate anions by ethylenediaminetetra acetic acid and 1,2-dihydroxyanthraquinone. J. Am. Chem. Soc. 95, 3564–3568.
LeeR B and RatcliffeR G 1983 Development of an aeration system for use in plant tissue NMR experiments. J. Exp. Bot. 34, 1213–1221.
LeonardR T and HansonJ B 1972 Increased membrane-bound ATPase activity accompanying development of enhanced solute uptake in washed corn root tissue. Plant Physiol. 49, 436–440.
LoughmanB C, RatcliffeR G and SchwabeJ W R 1989 Galactose metabolism inZea mays root tissues observed by31P NMR spectroscopy. Plant Sci. 59, 11–23.
LoughmanB C and WhiteP J 1984 The role of minor nutrients in the control of ion movement across membranes.In Membrane Transport in Plants. Eds. AWalker and JCram. pp 501–502. Academia, Prague.
MacaraI G, KustinK and CantleyL C 1980 Glutathione reduces cytoplasmic vanadate. Mechanism and physiological implications. Biochim. Biophys. Acta 629, 95–106.
PerlinD S and SpanswickR M 1981 Characteristics of ATPase activity associated with corn leaf plasma membranes. Plant Physiol. 68, 521–526.
PetersonG L 1977 A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83, 346–356.
PetersonG L 1978 A simplified method for analysis of inorganic phosphate in the presence of interfering substances. Anal. Biochem. 84, 164–172.
RossH H 1976 Theory and application of Ĉerenkov counting.In Liquid Scintillation Science and Technology. Eds. A ANoujaim, CEdiss and L IWiebe. pp 79–91. Academic Press, New York.
SzeH and HodgesT K 1977 Selectivity of alkali cation influx across the plasma membrane of oat roots. Cation specificity of the plasma membrane ATPase. Plant Physiol. 59, 641–646.
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Sklenar, J., Fox, G.G., Loughman, B.C. et al. Effects of vanadate on the ATP content, ATPase activity and phosphate absorption capacity of maize roots. Plant Soil 167, 57–62 (1994). https://doi.org/10.1007/BF01587598
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DOI: https://doi.org/10.1007/BF01587598