Skip to main content
Log in

Effect of amendments, moisture and temperature on acetylene reduction in nile delta soils

  • Published:
Plant and Soil Aims and scope Submit manuscript

Summary

The pattern of N2-ase activity in clay-loam soil of Nile Delta was determined. However, unamended soil showed somewhat low activity: an amount of 18–95 mg N2 fixed/kg soil/year was calculated. Addition of glucose greatly enhanced such activity and efficiencies of N2-fixation increased with decreasing carbon source concentration. Highest activities (800 n moles C2H4/gh−1) and efficiencies (18.06 mg N2/g glucose added) were reported in soil amended with 1% glucose, adjusted to 50% W.H.C. and incubated at 30°C. Enrichment of the soil with straw lead to a significant nitrogen gain particularly under water-logged conditions. During a short period of 16 days 5.8–9.3 mg N2 were fixed/g straw added at the latter conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Abd-El-Malek, Y. 1971 Free-living nitrogen-fixing bacteria in Egyptian soils and their poisible contribution to soil fertility. Plant and Soil Spec. Vol. 423–442.

  2. Barrow, N. J. and Jenkinson, N. S. 1962 The effect of waterlogging on fixation of nitrogen by soil incubated with straw. Plant and Soil16, 258–262.

    Google Scholar 

  3. Brouzes, R., Mayfield, C. I. and Knowles, R. 1971 Effect of oxygen partial pressure on nitrogen fixation and acetylene reduction in a sandy loam soil amended with glucose. Plant and Soil Spec. Vol. 481–494.

  4. El-Hadidy, Tomader 1965 Levels of Fertility and their Relation to Soil Microorganisms in Semi-arid Soils with Special Reference to Non-symbiotic Nitrogen Fixation; Ph.D. Thesis, Fac. Agr., Cairo University.

  5. Hardy, R. W. F., Holsten, R. D., Jackson, E. K. and Burns, R. C. 1968 The acetylene-ethylene assay for N2-fixation: Laboratory and field evaluation. Plant Physiol.43, 1185–1207.

    Google Scholar 

  6. Hegazi, N. A. 1969 Aerobic Non-symbiotic N2-fixing Bacteria in Egypt. M.Sc. Thesis, Fac. Agr., Cairo University.

  7. Hegazi, N. A. and Niemela, S. 1976 A note on the estimation ofAzotobacter densities by membrane filter technique. J. Appl. Bacteriol.41, 311–313.

    Google Scholar 

  8. Jensen, H. L. 1940 Nitrogen fixation and cellulose decomposition by soil microorganisms. I. Aerobic cellulose decomposers in association withAzotobacter. Proc. Linn. Soc. N.S.W.65, 543–556.

    Google Scholar 

  9. Jensen, H. L. 1965 Non-symbiotic nitrogen fixation.In Soil Nitrogen, eds. W. V. Bartholomew and F. E. Clark. Am. Soc. Agron. Monogr.10, 436–480.

  10. Kandil, M. F., Shawky, M. E., Abd El Hadi, J. and Fathi, A. 1976 Effect of soil texture on the available water of some soils from Egypt. Egypt J. Soil Sci.16, 153–161.

    Google Scholar 

  11. Magdoff, F. R. and Bouldin, D. R. 1971 Nitrogen fixation in submerged soil-sandy-energy media and the aerobic-anaerobic interface. Plant and Soil33, 49–61.

    Google Scholar 

  12. Mayfield, C. I. and Aldworth, R. L. 1974 Acetylene reduction in artificial soil aggregates amended with cellulose, wheat straw and xylan. Can. J. Microbiol.20, 1503–1507.

    Google Scholar 

  13. Mulder, E. G. and Brotonegoro, S. 1974 Free-living heterotrophic nitrogen-fixing bacteria.In The Biology of Nitrogen Fixation. Ed. A. Quispel (North-Holland-American Elsevier) 37–85.

  14. Okafor, N. and Macrae, I. C. 1973 The influence of moisture level, light, aeration and glucose upon acetylene reduction by a black earth soil. Soil Biol. Biochem.5, 181–186.

    Google Scholar 

  15. O'Toole, P. and Knowles, R. 1973 Efficiency of acetylene reduction (nitrogen fixation) in soil: Effect of type and concentration of available carbohydrate. Soil Biol. Biochem.5, 789–797.

    Google Scholar 

  16. Paul, E. A., Myers, R. J. K. and Rice, W. A. 1971 Nitrogen fixation in grassland and associated cultivated ecosystems. Plant and Soil, Spec. Vol. 495–507.

  17. Pochon, J. and Tardieux, P. 1962 Technique d'Analyse en Microbiologie du Sol. Ed. de la Tourelle, St. Mande, France.

    Google Scholar 

  18. Rajaramamohan-Rao, V. 1976 Nitrogen fixation as influenced by moisture content, ammonium sulfate and organic sources in a paddy soil. Soil Biol. Biochem.8, 445–448.

    Google Scholar 

  19. Rice, W. A. and Paul, E. A. 1972 The organisms and biological processes involved in asymbiotic nitrogen fixation in waterlogged soil amended with straw. Can. J. Microbiol.18, 715–723.

    Google Scholar 

  20. Shady, M. A. M. 1970 Microbiological Studies on Kharga Soils. M.Sc. Thesis. Fac. Agr., Cairo University.

  21. Vancura, V., Abd El Malek, Y. and Zayed, M. N. 1965 Azotobacter andBeijerinckia in the soils and rhizosphere of plants in Egypt. Folia Microbiol.10, 224–229.

    Google Scholar 

  22. Vlassak, K. and Jain, M. K. 1976 Biological nitrogen fixation in the rhizosphere ofChicorium intybus andTaraxacum officinale. Rev. Ecol. Biol. Sol13 411–418.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hegazi, N.A., Vlassak, K. & Monib, M. Effect of amendments, moisture and temperature on acetylene reduction in nile delta soils. Plant Soil 51, 27–37 (1979). https://doi.org/10.1007/BF02205924

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02205924

Key words

Navigation