Abstract
Chloroplast proteins were phosphorylated under two test conditions: ‘white light’ irradiance alone and ‘white light’ irradiance with the addition of glucose and glucose oxidase, used to produce an anaerobic medium. The interaction of phospho-LHC II with Photosystem 1 (PS 1) was studied for two types of PS I preparation. Changes in the chlorophyll a/b ratio and the ratio of 650 and 680 nm band intensities (E650/E680) in fluorescence excitation spectra were used in calculating the phospho-LHC II portion which became associated with PS 1. It is shown that the associated portion of phospho-LHC II varies for each of the PS 1 preparations and phosphorylation procedures. Possible conclusions as regards the transfer of various sets of LHC II subpopulations under different phosphorylation procedures and the differences of interaction with PS 1 are discussed.
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Abbreviations
- PS 1:
-
Photosystem 1
- PS 2:
-
Photosystem 2
- LHC II:
-
light-harvesting chlorophyll a/b protein complex II
- Chl:
-
chlorophyll
- ϕ:
-
fluorescence quantum yield
- τf :
-
life time of fluorescence at λ=685 nm
- F735:
-
fluorescence band with a maximum at 735 nm
- F685:
-
fluorescence band with a maximum at 685 nm
- E650/E680:
-
ratio of amplitudes in excitation fluorescence spectrum at 650 and 680 nm
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Kochubey, S.M., Shevchenko, V.V. & Volovik, O.I. Fluorescence studies on interaction between phospho-LHC II and subchloroplast Photosystem 1 preparations. Photosynth Res 38, 153–157 (1993). https://doi.org/10.1007/BF00146414
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DOI: https://doi.org/10.1007/BF00146414