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In vitro regeneration of Gaillardia pulchella Foug.

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Abstract

Young leaf and internodal stem segments of Gaillardia pulchella, collected from wild species re-established in the greenhouse, were used to initiate callus on Murashige & Skoog medium supplemented with NAA (2.0 mgl−1) and BA (0.4 mgl−1). Callus formed after 10 to 14 days in the dark. Cultures were transferred to fresh medium and placed under lighted conditions where shoot formation occurred approximately 14 to 30 days after initiation. Callus sub-cultured at 14 to 21-day intervals continued to produce primordia for several weeks. Flowers were produced by regenerated shoots maintained on MS medium, but roots did not develop until the plantlets were transferred to soil conditions.

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Abbreviations

NAA:

naphthaleneacetic acid

BA:

benzyladenine

GA3 :

gibberellic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige & Skoog medium

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Authors and Affiliations

  1. Department of Botany, University of Texas, 78713, Austin, Texas, USA

    J. E. Bourque, S. Tanner & T. J. Mabry

Authors
  1. J. E. Bourque
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  2. S. Tanner
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  3. T. J. Mabry
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Bourque, J.E., Tanner, S. & Mabry, T.J. In vitro regeneration of Gaillardia pulchella Foug.. Plant Cell Tiss Organ Cult 16, 67–72 (1989). https://doi.org/10.1007/BF00044074

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  • DOI: https://doi.org/10.1007/BF00044074

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