Abstract
Organogenic callus cultures of seashore mallow, Kosteletzkya virginica (L.) Presl., originated from excised mature embryos or stem sections of aseptically germinated plants initially cultured on Murashige & Skoog minimal organics medium containing 30000 mg l-1 glucose, 2.0 mg l-1 indoleacetic acid and 1.0 mg l-1 kinetin. Plants were regenerated via shoots and roots from callus cultures following transfer through a series of media with different cytokinin/auxin ratios and changes in carbohydrate source. Meristematic regions, shoot and root primordia were observed during histological examination of the tissues. Somatic embryos were not found.
Similar content being viewed by others
References
Brown DCW, Thorpe TA (1986) Plant regeneration by organogenesis. In: Vasil IK (Ed) Cell Culture and Somatic Cell Genetics of Plants (pp 49–60) Academic Press, New York.
Cook DA, Gallagher JL (1988) Protein production patterns of the brackish marsh dicot Kosteletzkya virginica (Malvaceae) change in response to salt challenge (in review).
Davidonis GH, Hamilton RH (1983) Plant regeneration from callus tissue of Gossypium hirsutum L. Plant Sci Lett 32:89–93.
Dubinski BJ (1987) Resource allocation within Kosteletzkya virginica L. Presl. (Malvaceae). Doctoral Dissertation. University of Delaware, College of Marine Studies. Lewes, DE, USA.
Finer JJ, Smith RH (1984) Initiation of callus and somatic embryos from explants of mature cotton (Gossypium klotzschianum Andress). Plant Cell Rep 3:41–43.
Gallagher JL (1985) Halophytic crops for cultivation at seawater salinity. Plant Soil 89:323–336.
Gawel NJ, Rao AP, Robacker CD (1986) Somatic embryogenesis from leaf and petiole callus cultures of Gossypium hirsutum L. Plant Cell Rep 5:457–459.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497.
Phillipp KR (1988) Water and salt budgets of selected halophytes. Doctoral Dissertation. University of Delaware, College of Marine Studies. Lewes, DE, USA.
Poljakoff-Mayber A, Somers GF, Werker E, Gallagher JL (1988) Seeds of Kosteletzkya virginica L. Presl., their structure, germination and salt tolerance (in review) Am J Bot.
Price HJ, Smith RH (1979) Somatic embryogenesis of suspension cultures of Gossypium hirsutum Andress. Planta 145:305–307.
Price HJ, Smith RH (1983) Cotton. In: Ammirato PV, Evans DA, Sharp WR, Yamada Y (Eds). Handbook of Plant Cell Culture (pp 487–510) Macmillan, New York.
Rangan TS, Zavala T, Ip A (1984) Somatic embryogenesis in tissue cultures of Gossypium hirsutum L. In Vitro 20:256.
Reynolds BD, Blackmon WJ (1983) Embryogenesis and plantlet regeneration from callus of Hibiscus acetosella. J Amer Soc Hort Sci 108:307–310.
Rumer S (1982) A comparison of fluctuating vs constant salinity stress on plants. Masters Thesis. University of Delaware, College of Marine Studies, Lewes, DE, USA.
Shoemaker RC, Crouche LJ, Galbraith DW (1986) Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep 3:178–181.
Smith RH, Price HJ, Thaxton JB (1977) Defined conditions for the initiation and growth of cotton callus in vitro. I. Gossypium arboreum. In Vitro 13:329–334.
Somers GF (1979) Natural halophytes as a potential resource for new salt tolerant crops: some progress and prospects. In: Hollaender A (Eds) The Biosaline Concept: An Approach to the Utilization of Underexploited Resources (pp 101–115) Plenum Press, New York.
Straub PF, Gallagher JL (1987) SDS soluble protein patterns in salt (NaCl) treated suspension cultures of three species of halophytes. J Cell Biochem 11B:45.
Tetu T, Sangwan RS, Sangwan-Norreel BS (1987) Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus. J Exp Bot 38:506–517.
Trolinder NL, Goodin JR (1985) Somatic embryogenesis in cell suspension cultures of Gossypium hirsutum. Beltwide Cotton Production Research Conference, Proceedings (p 46).
Trolinder NL, Goodin JR (1988) Somatic embryogenesis in cotton (Gossypium). I. Effects of source of explant and hormone regime. Plant Cell Tiss Org Cult 12:31–42.
Trolinder NL, Goodin JR (1988) Somatic embryogenesis in cotton (Gossypiun). II. Requirements for embryo development and plant regeneration. Plant Cell Tiss Org Cult 12:43–54.
Wright MS, Carnes MG, Hinchee MA, Davis GC, Koehler SM, Williams MH, Colburn SM, Pierson PE (1986) Plant regeneration from tissue cultures of soybean by organogenesis. In: Vasil IK (Ed) Cell Culture and Somatic Cell Genetics of Plants (pp 111–119) Academic Press, New York.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cook, D.A., Decker, D.M. & Gallagher, J.L. Regeneration of Kosteletzkya virginica (L.) Presl. (Seashore Mallow) from callus cultures. Plant Cell Tiss Organ Cult 17, 111–119 (1989). https://doi.org/10.1007/BF00046856
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00046856