Abstract
Somatic embryos and adult plants were regenerated from mesophyll protoplasts of a clone of chicory ‘474’ (Cichorium intybus L. x Cichorium endivia L.). Embryos were obtained in three different ways:
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- plating of 7-day-old protoplast cultures on PM-3 solid induction medium with 2.5 μM 2-isopentenyladenine (2-iP) and 0.5 μM naphthaleneacetic acid (NAA);
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- transfer of 1–2 mm microcolonies into liquid M-17 induction medium with 2.5 μM (2-iP) and 0.1 μM (NAA);
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- transfer of well-established protoplast-derived calluses onto M-17.
Development of embryos was accomplished in agitated liquid Heller medium with 0.15 μM gibberellic acid and germination on solid Heller medium without growth regulators. The total time for plantlet (4-leaves stage) recovery, following protoplast isolation, was 13 to 15 weeks. After acclimatization all protoplast-derived plants of Cichorium ‘474’ were phenotypically normal and fertile.
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Abbreviations
- GA3 :
-
gibberellic acid
- 2-iP:
-
2-isopentenyladenine
- NAA:
-
naphthaleneacetic acid
- P.E.:
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plating efficiency
- PM:
-
protoplast medium
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Sidikou-Seyni, R., Rambaud, C., Dubois, J. et al. Somatic embryogenesis and plant regeneration from protoplasts of Cichorium intybus L. x Cichorium endivia L.. Plant Cell Tiss Organ Cult 29, 83–91 (1992). https://doi.org/10.1007/BF00033612
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DOI: https://doi.org/10.1007/BF00033612