Abstract
This review describes recent advances in alternative DNA-delivery techniques with particular emphasis on silicon carbide fibers, intact tissue electroporation, electrophoresis and microinjection. The advantages/disadvantages of each method along with a historical overview and theory of practice are discussed.
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References
Abdul-Baki AA, Saunders JA, Matthews BF & Pittarelli GW (1990) DNA uptake during electroporation of germinating pollen grains. Plant Sci. 70: 181–190
Abdullah R, Cocking EC & Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Biotechnology 4: 1087–1090
Ahokas H (1989) Transfection of germinating barley seed electrophoretically with exogenous DNA. Theor. Appl. Genet. 77: 469–472
Akella V & Lurquin PF (1993) Expression in cowpea seedlings of chimeric transgenes after electroporation into seed-derived embryos. Plant Cell Rep. 12: 110–117
Anonymous (1994) Silicon carbide pokes into DNA. New Scientist 1909: 19
Antonelli NM & Stadler J (1990) Genomic DNA can be used with cationic methods for highly efficient transformation of maize protoplasts. Theor. Appl. Genet. 80: 395–401
Appel JD, Fasy TM, Kohtz DS & Johnson EM (1988) Asbestos fibers mediate transformation of moneky cells by exogenous plasmid DNA. Proc. Natl. Acad. Sci. 85: 7670–7674
Asano Y, Otsuki Y & Ugacki M (1991) Electroporation mediated- and silicon carbide fiber-mediated DNA delivery in Agrostis alba L. (Red top). Plant Sci. 79: 247–252
Bajaj YPS (1977) Protoplast isolation, culture and somatic hybridization. In: Reinert J & Bajaj YPS (Eds) Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture (pp 467–495). Springer Verlag, Berlin
Barnason A, Armstrong C, Dean D, Deaton R, Fischhoff D, Fromm M, Horsch R, Lavallee B, Maher G, Petersen W & Simms S (1991) Production of transgenic corn plants resistant to the European corn borer. Agronomy Abstract (p 192)
Bidney D, Scelonge C, Martich J, Burrus M, Sims L & Huffman G (1992) Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens. Plant Mol. Biol. 18: 301–313
Bower R & Birch RG (1992) Transgenic sugarcane plants via microprojectile bombardment. Plant J. 2: 409–416
Bradley PM (1979) Micromanipulation of cyanelles and a cyanobacterium into higher plant cells. Physiol. Plant. 46: 293–298
Bytebier B, Deboeck F, Greve HD, Van Montagu M & Hernalsteens JP (1987) T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis. Proc. Natl. Acad. Sci. 84: 5345–5349
Cao J, Duan X, McElroy D & Wu R (1992) Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Rep. 11: 586–591
Capecchi M (1980) High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell 22: 479–488
Casas AM, Kononowicz AK, Zehr UB, Tomes DT, Axtell JD, Butler LG, Bressan RA & Hasegawa PM (1993) Transgenic sorghum plants via microprojectile bombardment. Proc. Natl. Acad. Sci. 90: 11212–11216
Chan MT, Chang HH, Ho SL, Tong WF & Yu SM (1993) Agrobacterium-mediated production of transgenic rice plants expressing a chimeric α-amylase promoter/β-glucuronidase gene. Plant Mol. Biol. 22: 491–506
Chasan R (1992) Transforming maize transformation. Plant Cell 4: 1463–1464
Chowria G, Akelle V & Lurquin PF (1993) Transformation of peas and lentils by in vivo electroporation of nodal meristems. Western Society of Crop Science Abstracts (p 2)
Christou P, Ford TL & Kofron M (1991) Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Biotechnology 9: 957–962
Cocking EC (1960) A method for isolation of plant protoplasts and vacuoles. Nature 187: 962–963
Cocking EC (1972) Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23: 29–50
Constabel F (1990) Medicinal plant biotechnology. Planta Med. 56: 421–425
Crossway A, Oakes JV, Irvine JM, Ward B, Knauf VC & Shewmaker CK (1986) Intergration of foreign DNA following microinjection of tobacco mesophyll protoplasts. Mol. Gen. Genet. 202: 179–185
Dekeyser RA, Claes B, De Rycke RMU, Habets ME, Van Montagu MC & Caplan AB (1990) Transient gene expression in intact and organized rice tissues. Plant Cell 2: 591–602
DeLaat A & Blaas J (1987) An improved method for protoplasts microinjection suitable for transfer of entire plant chromosomes. Plant Sci. 50: 161–169
D'Halluin K, Bonne E, Bossut M, De Beuckeleer M & Leemans J (1992) Transgenic maize plants by tissue electroporation. Plant Cell 4: 1495–1505
Dhir SK, Dhir S & Widholm JM (1991) Plantlet regeneration from immature cotyledon protoplasts of soybean (Glycine max L) (Merr.). Plant Cell Rep. 10: 39–43
Draper J, Davey MR, Freeman JP, Cocking EC & Cox BJ (1982) Ti plasmid homologous sequences present in tissues from Agrobacterium plasmid-transformed Petunia protoplasts. Plant Cell Physiol. 23: 451–458
Dunahay TG (1993) Transformation of Chlamydomonas reinhardtii with silicon carbide whiskers. Biotechniques 15: 452–460
Finer JJ, Vain P, Jones MW & McMullen MD (1992) Development of the particle inflow gun for DNA delivery to plant cells. Plant Cell Rep. 11: 323–328
Fraley RT, Rogers SC, Horsch RB, Sanders PR, Flick JS, Fink C, Hoffman N & Sanders P (1983) Expression of bacterial genes in plant cells. Proc. Natl. Acad. Sci. 80: 4803–4807
Fromm ME, Taylor LP & Walbot V (1985) Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc. Natl. Acad. Sci. 82: 5824–5828
Fromm ME, Taylor LP & Walbot V (1986) Stable transformation of maize after gene transfer by electroporation. Nature 319: 791–793
Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J & Klein TM (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Biotechnology 8: 833–839
Fujimura T, Sakurai M, Akagi H, Negiski T & Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult. Lett. 2: 74–75
Godwin ID, Ford-Lloyd BV & Newbury HJ (1992) In vitro approaches to extending the host-range of Agrobacterium for plant transformation. Aust. J. Bot. 40: 751–763
Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams Jr. WR, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP & Lemaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2: 603–618
Grant JE, Dommisse EM, Christey MC & Conner AJ (1991) Gene transfer to plants using Agrobacterium. In: Murray DR (Ed) Advanced Methods in Plant Breeding and Biotechnology (pp 50–73). CAB International, Oxford, UK
Griesbach RJ & Hammond J (1993) Incorporation of the GUS gene into orchids via embryo electrophoresis. Acta. Hort. 336: 165–169
Griesbach RJ & Sink KC (1983) Evacuation of mesophyll protoplasts. Plant Sci. Lett. 30: 297–301
Griesbach RJ (1985) Advances in the microinjection of higher plant cells. Biotechniques 3: 348–350
Griesbach RJ (1987) Chromosome-mediated transformation via microinjection. Plant Sci. 50: 69–77
Gurdon J (1977) Methods for nuclear transplantation. Methods of Cell Biology 14: 125–139
Hagio T, Blowers AD & Earle ED (1991) Stable transformation of sorghum cell cultures after bombardment with DNA-coated microprojectiles. Plant Cell Rep. 10: 260–264
Hess D, Dressler K & Nimmrichter R (1990) Transformation experiments by pipetting Agrobacterium into the spikelets of wheat (Triticum aestivum L.). Plant Sci. 72: 233–244
Hinchee MAW, Connor-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischhoff DA, Re DB, Fraley RT & Horsch RB (1988) Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer. Biotechnology 6: 915–920
Horsch RB, Fry JE, Hoffman NL, Wallroth M, Eicholtz D, Rogers SG & Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231
Jaenisch R & Mintz B (1974) Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocytes injected with viral DNA. Proc. Natl. Acad. Sci. USA 71: 1250–1254
Jefferson RA, Kavanagh TA & Bevan MW (1987) GUS fusions: B-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901–3907
Joersbo M, Jorgensen RB & Olesen P (1990) Transient electropermeabilization of barley (Hordeum vulgare L.) microspores to propidium iodide. Plant Cell Tiss. Org. Cult. 23: 125–129
Jones H, Tempelaar MJ & Jones MGK (1987) Recent advances in plant electroporation. Oxford Surveys of Plant Mol. & Cell Biol. 4: 347–357
Kaeppler HF & Somers DA (1994) DNA delivery into maize cell cultures using silicon carbide fibers. In: Freeling M & Walbot V (Eds) The Maize Handbook (pp 610–613). Springer Verlag, New York
Kaeppler HF, Gu W, Somers DA, Rines HW & Cockburn AF (1990) Silicon carbide fiber-mediated DNA delivery into plant cells. Plant Cell Rep. 8: 415–418
Kaeppler HF, Somers DA, Rines HW & Cockburn AF (1992) Silicon carbide fiber-mediated stable transformation of plant cells. Theor. Appl. Genet. 84: 560–566
Klein TM, Wolf ED, Wu R & Sanford JC (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70–73
Klein TM, Harper EC, Svab Z, Sanford JC, Fromm ME & Maliga P (1988) Stable genetic transformation of intact Nicotiana cells by the particle bombardment process. Proc. Natl. Acad. Sci. 85: 8502–8505
Klein TM, Kornstein L, Sanford JC & Fromm ME (1989) Genetic transformation of maize cells by particle bombardment. Plant Physiol. 91: 440–444
Klenchin VA, Sukharen SI, Serov SM, Chernomordik LV & Chizmadzhev YA (1991) Electrically induced DNA uptake by cells in a fast process involving DNA electrophoresis. Biophys. J. 60: 804–811
Kloti A, Inglesias VA, Wunn J, Burkhardt PK, Datta SK & Potrykus I (1993) Gene transfer by electroporation into intact scutellum cells of wheat embryos. Plant Cell Rep. 12: 671–675
Koziel MG, Beland GL, Bowman C, Carozzi NB, Crenshaw R, Crossland L, Dawson J, Desai N, Hill M, Kadwell S, Launis K, Lewis K, Maddox D, McPherson K, Meghji MR, Merlin E, Rhodes R, Warren GW, Wright M & Evola SV (1993) Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Biotechnology 11: 194–200
Kranz E & Lorz H (1990) Micromanipulation and in vitro fertilization with single pollen grains of maize. Sex. Plant Reprod. 3: 160–169
Krens FA, Molendijk L, Wullems GJ & Schilperoort RA (1982) In vitro transformation of plant protoplasts with Ti-plasmid DNA. Nature 296: 72–74
Lawrence WA & Davies DR (1985) A method for the microinjection and culture of protoplasts at very low densities. Plant Cell Rep. 4: 33–35
Li BJ, Xu XP, Shi HP & Ke XY (1991) Introduction of foreign genes into the seed embryo cells of rice by electro-injection and the regeneration of transgenic rice plants. Sci. China 34: 923–930
Li L, Qu R, de Kochko A, Fauquet C & Beachy RN (1993) An improved rice transformation system using the biolistic method. Plant Cell Rep. 12: 250–255
Lindsey K (1992) Genetic manipulation of crop plants. J. Biotechnol. 26: 1–28
Lorz H, Baker B & Schell J (1985) Gene transfer to cereal cells mediated by protoplast transformation. Mol. Gen. Genet. 199: 178–182
Lorz H, Paszkowski J, Dierks-Ventling C & Potrykus I (1981) Isolation and characterization of cytoplasts and miniprotoplasts derived from protoplasts of cultured cells. Physiol. Plant. 53: 385–391
Lu G & Ferl RJ (1992) Site-specific oligodeoxynucleotide binding to maize Adh1 gene promoter represses Adh1-GUS gene expression in vivo. Plant Mol. Biol. 19: 715–723
Luo Z & Wu R (1988) A simple method for the transformation of rice via the pollen tube pathway. Plant Mol. Biol. Rep. 6: 165–174
Marsh WA, Maddock SE, Christensen RJ, Jiang XQ, Roth BA, Daywalt MJ, Kulisek ES, Winter KRK, Heiden RA, Wilkinson DR, Dolezal WE & Anderson BM (1993) Coat protein mediated virus resistance in transgenic maize. In Vitro Abstract 92A
Matthews BF, Abdul-Baki AA & Saunders JA (1990) Expression of a foreign gene in electroporated pollen grains of tobacco. Sex. Plant Reprod. 3: 147–151
McKnight TD, Lillis MT & Simpson RB (1987) Segregation of genes transferred to one plant cell from two separate Agrobacterium strains. Plant Mol. Biol. 8: 439–445
Mishra KP, Joshua DC & Bhatia CR (1987) In vitro electroporation of tobacco pollen. Plant Sci. 52: 135–139
Morikawa H & Yamada Y (1985) Capillary microinjection into protoplasts and intranuclear localization of injected materials. Plant Cell Physiol. 26: 229–236
Morrish F, Songstad DD, Armstrong CL & Fromm M (1993) Microprojectile bombardment: A method for the production of transgenic cereal crop plants and the functional analysis of genes. In: Hiatt A (Ed) Transgenic Plants Fundamentals and Applications (pp 133–171). Marcel Dekker, New York
Murry LE, Elliot LG, Capitant SA, West JA, Hanson KK, Scarafia L, Johnston S, DeLuca-Flaherty C, Nichols S, Cunanan D, Dietrich PS, Mettler IJ, Dewald S, Warnick DA, Rhodes C, Sinibaldi RM & Brunke KJ (1993) Transgenic corn plants expressing MDMV strain B coat protein are resistant to mixed infections of maize dwarf mosaic virus and maize chlorotic virus. Biotechnology 11: 1559–1564
Negrutiu I, Shillito R, Potrykus I, Biasini G & Sala F (1987) Hybrid genes in the analysis of transformation conditions. I. Setting up a simple method for direct gene transfer in plant protoplasts. Plant Mol. Biol. 8: 363–367
Neuhaus G, Spangeberg G, Mittelsten-Scheid O & Schweizer HG (1987) Transgenic rape seed plants obtained by the microinjection of DNA into microspore derived embyoids. Theor. Appl. Genet. 75: 30–36
Oard JH, Paige DF, Simmonds JA & Gradziel TM (1990) Transient gene expression in maize, rice, and wheat cells using an airgun apparatus. Plant Physiol. 92: 334–339
Oard J (1993) Development of an airgun device for particle bombardment. Plant Cell Tiss. Org. Cult. 33: 247–250
Paszkowski J, Shilito RD, Saul M, Mandak V, Hohn T, Hohn B & Potrykus I (1984) Direct gene transfer to plants. EMBO J. 3: 2717–2722
Penza R, Akella V & Lurquin PF (1992) Transient expression and histological localization of a GUS chimeric gene after direct transfer to mature cowpea embryos. Biotechniques 13: 576–579
Potrykus I, Paszkowski J, Saul NW, Negrutiu I & Shillito RD (1987) Direct gene transfer to plants: facts and future. In: Green CE, Somers DA, Hackett WP & Biesboer DD (Eds) Plant Tissue and Cell Culture (pp 298–302). Alan R. Liss Inc, New York
Potrykus I, Saul M, Paskowski J & Shillito RD (1985) Direct gene transfer into protoplasts of a graminaceous monocot. Mol. Gen. Genet. 199: 183–188
Potrykus I (1991) Gene transfer to plants. Assessment of published approaches and results. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42: 205–225
Prioli LM & Sondahl MR (1989) Plant regeneration from protoplasts isolated from embryogenic maize cell cultures. Biotechnology 7: 589–594
Reich TJ, Iyer VN & Miki BL (1986b) Efficient transformation of alfalfa protoplasts by the intranuclear microinjection of Ti plasmids. Biotechnology 4: 1001–1004
Reich TJ, Iyer VN, Scobie B & Miki BL (1986a) A detailed procedure for the intranuclear microinjection of plant protoplasts. Can. J. Bot. 64: 1255–1258
Rhodes CA, Lowe KS & Ruby KL (1988a) Plant regeneration from protoplasts isolated from embryogenic maize cell cultures. Biotechnology 6: 56–60
Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D & Detmer JJ (1988b) Genetically transformed maize plants from protoplasts. Science 24: 204–207
Russell JA, Roy MK & Sanford JC (1992) Physical trauma and tungsten toxicity reduce the efficiency of biolistic transformation. Plant Physiol. 98: 1050–1056
Russell-Kikkert J (1993) The Biolistic PDS-1000/He device. Plant Cell Tiss. Org. Cult. 33: 221–226
Sahi SV, Chilton MD & Chilton WS (1990) Corn metabolites affect growth and virulence of Agrobacterium tumefaciens. Proc. Natl. Acad. Sci. 87: 3879–3883
Sanders LC & Lord EM (1989) Directed movement of latex particles in the gynoecia of three species of flowering plants. Science 243: 1606–1608
Shillito RD, Carswell GK, Johnson CM, DiMaio JJ & Harms CT (1989) Regeneration of fertile plants from protoplasts of elite inbred maize. Biotechnology 7: 581–587
Shillito RD, Saul MW, Paskowski J, Muller M & Potrykus I (1985) High efficiency direct gene transfer to plants. Biotechnology 3: 1099–1103
Shimamoto K, Terada R, Izawa T & Fujimoto H (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338: 274–276
Simmonds J, Stewart P & Simmonds D (1992) Regeneration of Triticum aestivum apical explants after microinjection of germ line progenitor cells with DNA. Physiol. Plant. 85: 197–206
Somers DA, Rines HW, Gu W, Kaeppler HF & Bushnell WR (1992) Fertile, transgenic oat plants. Biotechnology 10: 1589–1594
Songstad DD, Hairston BM & Armstrong CL (1993a) Stable transformation of maize by microprojectile bombardment of immature embryos. Agronomy Abstract (p 183)
Songstad DD, Halaka FG, DeBoer DL, Armstrong CL, Hinchee MAW, Ford-Santino CG, Brown SM, Fromm ME & Horsch RB (1993b) Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation. Plant Cell Tiss. Org. Cult. 33: 195–201
Songstad DD, Lowe KL, Betz SR & Cabrera-Ponce JL (1992) Callus cultures as alternative target tissues in microprojectile-mediated transformation of maize. Agronomy Abstracts (p 198)
Spencer TM, Gordon-Kamm WJ, Daines RJ, Start WG & Lemaux PG (1990) Bialaphos selection of stable transformants from maize cell cultures. Theor. Appl. Genet. 79: 625–631
Steinbiss H & Stabel P (1983) Protoplasts derived tobacco cells can survive capillary microinjection of the fluorescent dye Luciefer Yellow. Protoplasma 116: 223–227
Takebe I, Labib G & Melchers G (1971) Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 58: 318–320
Takeuchi Y, Dotson M & Keen NT (1992) Plant transformation: a simple particle bombardment device based on flowing helium. Plant Mol. Biol. 18: 835–839
Thompson JA, Abdullah R & Cocking EC (1986) Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose. Plant Sci. 47: 123–134
Tomes DT, Weissinger AK, Ross M, Higgins R, Drummond BJ, Schaaf S, Malone-Schoneberg J, Staebell M, Flynn P, Anderson J & Howard J (1990) Transgenic tobacco plants and their progeny derived by microprojectile bombardment of tobacco leaves. Plant Mol. Biol. 14: 261–268
Toriyama KF, Arimoto Y, Uchimiya H & Hinata K (1988) Transgenic rice plants after direct gene transfer into protoplasts. Biotechnology 6: 1072–1074
Umbeck P, Johnson G, Barton K & Swain W (1987) Genetically transformed cotton (Gossypium hirsutum L.) plants. Biotechnology 5: 263–266
Vain P, Keen N, Murillo J, Rathus C, Nemes C & Finer JJ (1993a) Development of the particle inflow gun. Plant Cell Tiss. Org. Cult. 33: 237–246
Vain P, McMullen MDM & Finer JJ (1993b) Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize. Plant Cell Rep. 12: 84–88
Vasil IK (1987) Developing cell and tissue culture systems for the improvement of cereal and crop plants. J. Plant Physiol. 128: 193–218
Vasil V, Castillo AM, Fromm ME & Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Biotechnology 10: 667–674
Verpoorte R, van der Heijden R & Schripsema J (1993) Plant cell biotechnology for the production of alkaloids: present status and prospects. J. Nat. Prod. 56: 186–207
Wallin A, Glimeluis G & Erikson T (1978) Enucleation of plant protoplasts by cytochalasin B. Z. Pflanzenphysiol 87: 333–340
Walters DA, Vetsch CS, Potts DE & Lundquist RC (1992) Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Plant Mol. Biol. 18: 189–200
Wan Y & PG Lemaux (1994) Generation of large numbers of independently transformed fertile barley plants. Plant Physiol. 104: 37–48
Watanabe M & Yamaguchi H (1988) The methods for isolation of cytoplasts in several crop plants. Japan J. Breed. 38: 43–52
Weeks JT, Anderson OD & Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol. 102: 1077–1084
Zaghmout OMF & Trolinder NL (1993) Simple and efficient method for directly electroporating Agrobacterium plasmid DNA into wheat callus cells. Nucleic Acid Res. 21: 1048
Zimmerman U (1986) Electrical breakdown, electropermeabilization and electrofusion. Rev. Physiol. Biochem. Pharmacol. 105: 175–256
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Songstad, D.D., Somers, D.A. & Griesbach, R.J. Advances in alternative DNA delivery techniques. Plant Cell Tiss Organ Cult 40, 1–15 (1995). https://doi.org/10.1007/BF00041112
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DOI: https://doi.org/10.1007/BF00041112