Skip to main content
SpringerLink
Log in

Tissue culture and micropropagation of Cuphea ericoides, a potential source of medium-chain fatty acids

  • Research Note
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Plant rgeneration occurred on leaf-and stem-derived callus of Cuphea ericoides Cham. & Schlechtd obtained in Murashige and Skoog medium supplemented with auxins [indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-d)] plus cytokinins [6-benzyladenine (BA) or kinetin]. These calluses were subcultured and showed vigorous growth. When subcultured on medium containing 2.22 or 4.44 μM BA, the calluses showed profuse regeneration of shoots whereas those subcultured on medium supplemented with 2.69 μM NAA or 0.226 μM 2,4-d produced numerous roots. Isolated shoots rooted on Murashige and Skoog medium lacking growth regulators or containing 0.54 μM NAA or 0.49 μM indole-3-butyric acid (IBA). Plantlets were acclimatized to greenhouse conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzyladenine

2,4-d :

2,4-dichlorophenoxyacetic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige & Skoog medium

NAA:

1-α-naphthaleneacetic acid

References

  • Babayan VK (1981) Medium chain length fatty acid esters and their medical and nutritional applications. J. Amer. Oil Chem. Soc. 58: 49A-51A.

    Google Scholar 

  • Graham SA (1989) Cuphea: a new plant source of medium-chain fatty acids. Food Sci. Nutr. 28: 139–173.

    Google Scholar 

  • Graham SA & Kleiman R (1987) Seed lipids of the Lythraceae. Biochem. Syst. Ecol. 15: 433–439.

    Google Scholar 

  • Hirsinger F (1985) Agronomic potential and seed composition of Cuphea, an annual crop for lauric and capric seed oils. J. Amer. Oil Chem. Soc. 62: 76–80.

    Google Scholar 

  • Hirsinger F & Knowles PF (1984) Morphological and agronomic description of selected Cuphea germplasm. Econ. Bot. 38: 439–451

    Google Scholar 

  • Janick J & Whipkey A (1986) In vitro propagation of Cuphea wrightii. HortScience 21: 135–137

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19: 389–404

    Google Scholar 

  • Thompson AE (1984) Cuphea: a potential new crop. HortScience 19: 352–354

    Google Scholar 

  • Wolf RB, Graham SA & Kleiman R (1983) Fatty acids composition of Cuphea seed oils. J. Amer. Oil Chem. Soc. 60: 103–104

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. CENARGEN/EMBRAPA, C.P. 02372, Brasília, 70849-970, Brasil

    I. Rita

  2. Plant Cell Biology Laboratory, Dep. of Botany, Inst. of Biosciences, USP, C.P. 11461, 05422-970, São Paulo, Brasil

    E. I. S. Floh

Authors
  1. I. Rita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. I. S. Floh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rita, I., Floh, E.I.S. Tissue culture and micropropagation of Cuphea ericoides, a potential source of medium-chain fatty acids. Plant Cell Tiss Organ Cult 40, 187–189 (1995). https://doi.org/10.1007/BF00037674

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00037674

Key words

Search

Navigation